中文摘要：

采用高碘酸钠对海藻酸钠（SA）进行氧化得到氧化海藻酸钠（OSA）,以丁二酸酐对壳聚糖（SC）进行接枝改性得到N–琥珀酰壳聚糖（NSC）,并与OSA发生Schiff碱反应生成水凝胶。采用水热合成法制备了微米级、纳米级羟基磷灰石并进行相应表征,并与OSA、SA制备复合水凝胶。利用静电纺丝技术制备了聚ε–己内酯/聚乙二醇细胞分隔膜并表征其形貌特征。采用乳化剂交联法,制备了SA竖直贯通多孔支架并研究了SA浓度、复合纳米羟基磷灰石对支架的影响。通过模块化构建,制备出多层一体化组织工程支架,将各组分材料与人皮肤成纤维细胞共培养以考察支架的生物相容性,采用吖啶橙荧光染色法观察细胞生长情况,细胞增殖定量检测采用CCK–8表征。

英文摘要：

The oxidized sodium alginate （OSA） was obtained by oxidizing sodium alginate （SA） with sodium periodate. The N-succinyl chitosan （NSC） was obtained by graft modification of chitosan （SC） with succinic anhydride. The hydrogel was formed by Schiff base reaction between NSC and OSA. The micro grade and nano grade hydroxyapatites were prepared by using the hydrothermal synthesis method, and the corresponding characterization was carried out. The composite hydrogel was prepared by reaction of the hydroxyapatite with OSA and SA. The poly（ε-caprolactone）/polyethylene glycol cell separation membrane was prepared by using the electrostatic spinning technology, and its morphology characteristics were characterized. The SA vertical through porous scaffolds were prepared by using the emulsifier cross-linking method, and the influence of SA concentration, composite nano hydroxyapatite on the scaffords was investigated. The multi-layer integrated tissue engineering scaffolds were prepared by using the modular construction. The bio-compatibility of the scaffolds was investigated by co-culturing each component material with the human skin fibroblast. The cell growth case was observed by using the acridine orange fluorescent staining method. The quantitative detection of the cell proliferation was characterized bv CCK-8.