为了探讨丛枝菌根真菌(Arbuscular mycorrhizalfungi,AMF)对烤烟生长及磷石膏农用安全的影响,通过盆栽模拟试验研究了磷石膏(Phosphogypsum,PG)不同添加量[0、40mg·g^-1(PG0、PG40)1和接种两种AMF[Glomus massecle(GM)、G.aggregatum(GA)】对烤烟(KRK26)苗期生长及其磷(P)、硫(S)、砷(As)吸收的影响。试验结果表明:无论是否接种AMF,磷石膏的添加均显著增加了KRK26地上部生物量及其植株s含量、吸收量及吸收效率;除不接种处理(NM)的烤烟根系外,PG40处理显著增加了KRK26植株P含量、吸收量及吸收效率,并显著降低了NM处理的地上部As含量及吸收量,进而增加了磷砷吸收比。相同PG添加水平下,与不接种相比,接种GM和GA均显著增加了KRK26植株的生物量。除PGO处理的烤烟根系外,接种GM显著增加了KRK26植株P、S含量与吸收量及吸收效率,以及植株As含量及吸收量,并显著增加了PG40处理的植株磷砷吸收比;接种GA也显著增加了KRK26植株P、S含量及吸收量,并显著降低了PG0处理地上部As含量及吸收量。所有复合处理,以添加磷石膏40mg·g^-1和接种GA处理对KRK26的生长促进效果较好,对磷石膏施用造成的As污染有一定抵御作用。
Phosphogypsum( PG ), an industrial by-product, contains P, S As and other micronutrients. Its agricultural applications may cause soil and plant contamination. The objective of this study was to investigate the role of arbuscular mycorrhizal fungi (AMF) on tobacco( Nico- tiana tabacum L. ) growth and arsenic (As) accumulation under phosphogypsum(PG) treatments in a pot experiment. Two PG application levels(0 and 40 mg·g^-1) and two AMF species, i.e. Clornus rnosseae(GM) and G. aggregatum(GA), were used. Plant biomass and phos- phorus( P ), sulfur( S ), and As uptake of tobacco( KRK26 ) were measured. Regardless of. AMF inoculation, applying PG amendment signifi- cantly increased shoot biomass and tissue concentrations, total uptake and specific absorption rate of S in tobacco. Amendment of PG also significantly increased tissue concentrations, total uptake and specific absorption rate of P in tobacco, with an exception of roots in non-AMF ( NM ) treatment. However, PG amendment significantly decreased tissue concentrations and uptake of As in tobacco shoots with NM, and thus significantly increasing the absorption ratios of P to As. Under PG treatment, both GM and GA inoculations significantly increased plant hiomass of tobacco, compared with the NM treatment. GM inoculation also significantly increased the tissue concentrations and total uptakes of P, S, and As, as well as the specific absorption rates of P and S, excepting roots in non-PG(PG0) treatment. In addition, GM inoculation significantly increased the ratios of P to As absorption in tobacco with the PG40(40 mg PG·g^-1) treatment. Similarly, GA inoculation signifi- cantly increased tissue concentrations and total uptake of P and S, but significantly decreased tissue concentrations and total absorption of As with the PG0 treatment. Combination of PG40 and GA inoculation had the most desirable effects on tobacco growth and could reduce the risk of As pollution caused by agricultural application of P