采用A/O(水解酸化+生物接触氧化)工艺接种生活污水处理厂普通活性污泥,研究生物膜法处理高盐混合化工废水的启动过程及处理效果。结果表明,采用分段连续挂膜法,3周即可快速完成挂膜;采用分阶段逐步加压驯化法,第一阶段以难降解物质为选择压力,进水COD浓度1 500 mg/L时,系统出水COD为50 mg/L左右,COD平均去除率95%,A池VFA平均增长率259.4%,酸化率26.8%;第二阶段以盐分为选择压力,含盐量14 g/L时,出水COD浓度保持在220~269 mg/L,COD平均去除率83%,A池VFA平均增长率231.9%,酸化率19.9%;整体驯化完成后,生物膜活性良好,胞外多聚糖浓度为5.4 mg/g MLSS,脱氢酶活性为3.1μg TF/(m L·h);通过扫描电镜观察,A池生物膜以长短不一的杆状菌为主并伴有少量球菌,O池中丝状菌、各种杆菌、球菌以及胞外聚合物在填料表面形成致密的生物膜。
Bench scale experiment of treating mixed chemical wastewater of high salt content were conducted, adopting bio- film process which was inoculated with conventional WWTP activated sludge of A/O process. The results of the experiment showed that bio-film could be rather quickly formed in three weeks; the acclimation was gradually done by stages, i.e., at the first stage when refractory organics being the selection pressure, the effluent COD concentration was as low as ca. 50 mg/L, the influent COD concentration being 1 500 mg/L, thus the average removal was 95%, correspondingly, at the same time, the average growth rate of VFA in pool A was 259.4%, and the acidification rate was 26.8%; on the other hand, at the second stage when salt being the selection pressure and the salt content 14 g/L, the effluent COD concentration maintained at range from 220mg/L to 269mg/L, thus the average COD removal reached 83%,while the average growth rate VFA was 231.9%, and the acidification rate 19.9%. When acclimation was completed, the concentration of extraceIlular polysaccharides was 5.4 mg/g MLSS, dehydrogenase activity was 3.1 μgTF/(mL h). It was found by SEM that biofilm in pool A was composed mainly of bacillus of different lengths accompanied by some coccus; while in pool O the bio-film was formed by extracellular polymers, filamentous bacteria and various kinds of bacillus and coccus.