中文摘要：

目的通过测定并比较紫癜性肾炎（HSPN）患儿及健康儿童血清IgA1水平，探讨IgA1对HSPN发病的影响。方法收集2013年5-12月河南中医学院第一附属医院儿科住院的HSPN患儿40例（HSPN组）及20名健康儿童（对照组）献血者血清，采用免疫比浊法测定HSPN患儿及健康儿童血清IgA的浓度：通过Jacalin亲和层析法分离、纯化血清，得到Jacalin结合蛋白，测定两组血清总IgA1浓度；Jacalin结合蛋白通过分子筛层析法分离，测定两组单聚体IgA1（mIgA1）及多聚体IgA1（pIgA1）的浓度。结果HSPN组及对照组血清IgA水平比较，差异无统计学意义（P〉0．05）；HSPN组血清总IgA1、mIgA1及pIgA1水平均高于对照组，差异有高度统计学意义（P〈0．01）。结论HSPN患儿血清IgA水平升高不明显，血清中mlgA1及pIgA1水平明显升高．提示IgA1在HSPN的发病中起一定作用。

英文摘要：

Objective To measure and compare the differences of IgA1 serum level in children with henoch-schonlein purpura nephritis （HSPN） and healthy children （control group）, and discuss the impact on the disease. Methods Serums of children with HSPN （HSPN group） and healthy children from May to December 2013 in the First Affiliated Hospital of He＇nan University of TCM were collected. Concentration of serum IgA in two groups were measured by immunotur- bidimetry; Jacalin bound protein was separated and purified by Jacalin affinity chromatography, and concentration of to- tal serum IgA1 in two groups were measured; monomerie IgA1 and polymeric IgA1 were separated by molecular sieve chromatography and the concentration of mlgA1 and plgA1 of two groups were measured. Results There was no statisti- cally significant difference of two groups in the concentration of serum IgA （P 〉 0.05）; the concentration of total serum IgA1, mlgA1 and plgA1 in children with HSPN was significantly higher than those of the control group, the difference was high statistically significant （P 〈 0.01）. Conclusion The increase of the level of serum IgA in children with HSPN is not obvious, both mlgA1 and plgA1 are elevated significantly and they may play an important role in the pathogene- sis of HSPN.