中文摘要：

目的：探讨二甲双胍联合表皮生长因子受体（EGFR）信号通路阻断剂对子宫内膜癌细胞增殖的影响及相关的作用机制。方法：选取子宫内膜癌Ishikawa及HEC-1A细胞,CCK-8方法检测二甲双胍联合或者不联合EGFR信号通路抑制剂AG1478对子宫内膜癌细胞增殖的影响。RT-PCR检测二甲双胍对EGFR mRNA的影响。Western blotting检测二甲双胍作用下子宫内膜癌细胞磷酸化EGFR/总EGFR和磷酸化ERK/总ERK水平的变化。结果：1AG1478能够抑制子宫内膜癌细胞的增殖,二甲双胍能够增强其抑制作用;2二甲双胍抑制EGFR mRNA的表达;3二甲双胍能够抑制EGFR及ERK磷酸化蛋白的表达。结论：二甲双胍能够增强EGFR信号通路阻断剂AG1478对子宫内膜癌的抑制作用;并且二甲双胍可能通过抑制EGFR磷酸化蛋白的表达从而抑制ERK蛋白的磷酸化进而抑制子宫内膜癌细胞的增殖。

英文摘要：

Objective： To explore the effect of metformin combined with epidermal growth factor receptor （EGFR） signaling path- way inhibitor on proliferation of endometrial cancer cells and the related mechanism. Methods ： Endometrial cancer Ishikawa and HEC - 1A cells were selected. The effects of metformin combined with AG1478 and simple metformin on proliferation of endometrial cancer cells were detected by CCK - 8 method. The effect of metformin on EGFR mRNA was detected by RT - PCR. After treated with metformin, the changes of pEGFR/t - EGFR and pERK/t - ERK ratios in endometrial cells were detected by Western blotting. Results： AG1478 inhibited proliferation of endometrial cancer cells, metformin enhanced the effect. Metformin inhibited the expression of EGFR mRNA. Metformin inhibited the expressions of pEGFR and pERK. Conclusion ： Metformin can enhance the inhibitory effect of AG1478 on proliferation of endometrial cancer ceils. Metformin can significantly inhibit proliferation of endometrial cancer cells by inhibiting pEGFR expression and pERK expression.