中文摘要：

目的在血浆中筛选与大动脉炎疾病和时期相关的血浆差异表达蛋白。方法提取各20例大动脉炎急、慢性期患者和20例健康者的血浆进行蛋白双向凝胶电泳，计算机图像软件分析筛选差异性表达的蛋白点，基质辅助激光解析电离飞行时间（MALDI—TOF）质谱鉴定差异表达蛋白。并对部分蛋白采用酶联免疫吸附法（ELISA）检测血浆中的含量。结果筛选鉴定出疾病差异表达蛋白14种，包括血清淀粉样蛋白A，血清淀粉样P物质，纤维蛋白原，C3c，CAa，C7，C4结合蛋白，补体因子H．相关蛋白1，α－1酸性糖蛋白，重组激活基因蛋白1（RAG1），载脂蛋白A—I、IV，α1－微球蛋白，转甲状腺索蛋白，结合珠蛋白；其中与大动脉炎不同时期相关的蛋白有血清淀粉样蛋白A、纤维蛋白原、转甲状腺素蛋白、结合珠蛋白。在ELISA检测中，急性期患者血清中血清淀粉样蛋白A水平（中位数95．9mg／L）和显著高于慢性期（中位数49．2mg／L，P=0．009）和健康对照组（中位数23．9mg／L，P=0．001）。急性期补体C4结合蛋白CABP（中位数88．5mg／L）显著高于慢性期（中位数61．7mg／L，P=0．023）和正常对照（中位数32．6mg／L，P=0．001）。结论大动脉患者血浆有多种免疫有关蛋白和急性时相蛋白的表达，有可能用于疾病不同时期的判定；补体激活，补体调节蛋白和自身抗体的产生均参与大动脉炎的免疫病理机制，对这些蛋白的进一步研究有助于阐明大动脉炎的病理机制。

英文摘要：

Objectives To investigate differential expression of plasma proteins of patients with Takayasu＇s arteritis to screen disease-related or phase-related proteins or biomarkers. Methods From March, 2005 to January, 2006, Plasma of 20 patients with acute Takayasu＇s arteritis, 20 patients with chronic Takayasu＇s arteritis , and 20 healthy people as control were collected. Plasma proteins were profiled by two-dimensional eleetrophoresis. Spots of differential expression were screened by computerized map analysis and identified by matrix assisted laser desorption/ionization time of flight-mass spectrometry. Circulation levels of parts of differential expression proteins were investigated by Elisa in each subject. Results Fourteen differential expression proteins were identified, including serum amyloid A, serum amyloid P, fibrinogen, complement C3c, C7, CA binding protein, factor H related protein-l, immunoglobin,α-acid glycoprotein, RAGlprotein, α1-microglobin, apolipoprotein A-I,A-IV, transthyretin, haptoglobin. Proteins related to acute and chronic phase were serum amyloid A, fibrinogen, transthyretin, haptoglobin; Circulation levels of Serum amyloid A （SAA） and Complement CA binding protein （C4BP） were significantly increased in active TA patients comparing to that in inactive TA patients and in controls （SAA：95.9 vs 49.2, P =0.009 and 23.9 mg/L, P =0.001, respectively; CABP：88.5 vs 61.7, P= 0. 023 and 32. 6 mg/L, P 〈 0. 001, respectively）. Conclusions Acute phase proteins and immune proteins may possible be markers for diagnosis and activity of Takayasu＇s arteritis, Complement activity, complement modulation protein and antibody production may be involved in immune mechanism of Takayasu＇s arteritis. Further study of these proteins may be helpful to elucidate the pathologic mechanism of Takayasu＇s arteritis.