中文摘要：

目的：探讨野生型星形胶质细胞（ASC）和运动神经元VSC4.1（VSC）共培养时两者产生活性氧（ROS）的相互影响。方法：MTT法测定比较ASC和VSC在单独培养和共培养时受到兴奋性刺激后生长抑制率;ASC与Hoechst 33342标记的VSC共培养或分别单独培养时,激光共聚焦显微镜实时观察2种情况下两者产生ROS的能力。结果：较高浓度谷氨酸刺激可使共培养的混合细胞抑制率（IRVSC＋ASC）明显高于星形胶质细胞抑制率（IRASC）,而较低浓度时IRVSC＋ASC高于IRVSC。激光共聚焦显微镜实时观察发现共培养时VSC产生ROS水平明显低于单独培养的VSC,且前者在15min时出现明显升高,而单独培养的VSC产生的ROS在5min内达到峰值后即逐渐降低;共培养时ASC产生的ROS在10min时出现明显升高。结论：ASC和VSC共培养时ASC可降低VSC静息时的ROS水平,在受到兴奋性刺激后ASC延长VSC产生高水平ROS的时程。

英文摘要：

AIM ： To explore the mutual effect of co - culture of wild - type astrocytes （ASC） and motor neurons VSC4.1 （VSC） on the respective ability to produce reactive oxygen species （ROS）. METHODS： The inhibition rates of cell growth in ASC and VSC in co -culture or independent culture was detected after exposed to excitatory stimulus by MTr method. Real - time observation of ROS production by ASC and VSC labeled with Hoechst 33342 was detected by confocal microscopy under the conditions of co - culture or independent culture. RESULTS ： Higher concentration of glutamate induced a higher inhibition rate in mixed cell growth than that in ASC alone, while lower concentration of glutanlate induced a higher inhibition rate in mixed cell growth than that in VSC only. Real -time observation by confocal microscopy showed that ROS production by VSC under the condition of co - culture, which showed a notable increase at 15 min, was significant less than that in independent culture, which peaked at 5 min and was gradually decreased. ROS production by ASC in co - culture began to increase significantly at 10 rain. CONCLUSION： Compared to independent culture, ASC reduces the resting ROS production by co -cultured with VSC, while ASC prolongs the duration of ROS production by VSC after exposed to excitatory stimulus.