中文摘要：

目的：研究索拉菲尼（Sorafenib）诱导的自噬在肝癌细胞凋亡中的作用。方法：体外培养肝癌细胞Huh7和LH86细胞株。分为对照组、Sorafenib处理组、Bafilomycin处理组、Sorafenib＋Bafilomycin处理组。将构建的质粒表达载体pEGFP-N1-LC3分别转染Huh7和LH86细胞株,用索拉菲尼或者索拉菲尼加Bafilo-mycin同时处理细胞,通过荧光显微镜观察对照组和处理组细胞内细胞自噬小体形成情况。免疫印迹（West-ernblot）方法检测细胞自噬作用标志蛋白LC3以及细胞凋亡蛋白Caspase3切割条带在各组中的表达,Heochst33342染料对各组细胞核进行染色,以评估细胞自噬作用对索拉菲尼诱导细胞凋亡的影响。结果：在荧光显微镜下,可以观察到Sorafenib能够显著诱导两种肝癌细胞株发生自噬作用。抑制剂Balfilomycin能够显著抑制Sorafenib诱导的自噬作用。Balfilomycin抑制自噬能够显著增强Sorafenib诱导的细胞凋亡。结论：Sorafenib能够诱导肝癌细胞产生自噬作用。这种自噬作用在Sorafenib诱导的肝癌细胞凋亡中起到负调控作用。

英文摘要：

Objective：To investigate the roles of autophagy of Sorafenib - induced apoptosis in hepatocellular carcinoma （HCC） cells. Methods：Two cell lines including Huh7 and LH86 were cultured in vitro. We set up control group, Sorafenib -treated group, Bafilomycin -treated group and Sorafenib plus Bafilomycin -treated group. Cells untreated or treated with various conditions were observed under microscope to show autophasome formation. Western blot was performed to detect apoptotic marker molecule Caspase 3 cleavage activation and autophagy marker protein LC3. Results：Sub lethal Sorafenib induced apparent autophagy in Huh7 and LH86 cells. Inhibition of autophay by Bafilomycin significantly sensitized Sorafenibinduced apoptosis in HCC cells. Conclusion：Autophagy negatively regulates Sorafenib- induced apoptosis in HCC cells.