中文摘要：

发信号的分子刺猬(Hh ) 是必要的因为细胞的发信号在脊椎动物和无脊椎动物在胚胎、单个的开发为身体模式形成要求了。修正了 1 (Ptc1 ) ， Hh 的受体，调停由 binging 的 Hh 的活动 Hh ligands。在这研究，一半光滑的舌头脚底 Cynoglossus semilaevis (CsPtc1 ) 的 Ptc1 被克隆并且描绘。CsPtc1 cDNA 全身是 5 212 核苷酸(nt ) ，并且与 1 543 氨基酸残余编码蛋白质。CsPtc1 与另外的 telesot 种类的 Ptc1 分享了许多特征。实时量的 PCR 揭示了在大脑，肝，心，鳃，肠，怒气，性腺和肾表示的 CsPtc1 mRNA，与在比在卵巢显著地高的睾丸的表示水平。在睾丸，杂交信号主要在主要 spermatocytes，第二等的 spermatocytes，和 sertoli 房间被检测，当弱信号在卵母细胞被发现时。相应地， methylation 的度比在男性和 pseudomale 在女性是更高的。结果显示 CsPtc1 可以涉及男性和伪男性细菌线和精子发生的荒芜的刺猬(DHH ) 维护。

英文摘要：

The signaling molecule hedgehog（Hh） is essential for cellular signaling required for body pattern formation in embryonic and individual development in vertebrate and invertebrates.Patched 1（Ptc1）,a receptor of Hh,mediates the activity of Hh by binging the Hh ligands.In this study,the Ptc1 of half-smooth tongue sole Cynoglossus semilaevis（CsPtc1） was cloned and characterized.The full-length of CsPtc1 cDNA is 5 212 nucleotides（nt）,and encodes a protein with 1 543 amino acid residues.CsPtc1 shared many features with Ptc1 of other telesot species.Real-time quantitative PCR revealed CsPtc1 mRNA expressed in brain,liver,heart,gill,intestines,spleen,gonad and kidney,with expression level in testis significantly higher than in ovary.In testis,hybridization signals were mainly detected in primary spermatocytes,secondary spermatocytes,and sertoli cells,while weak signals were found in oocytes.Correspondingly,the degree of methylation is higher in female than in male and pseudomale.Results indicate that CsPtc1 may be involved in Desert Hedgehog（DHH） maintenance of the male and pseudo-male germ line and spermatogenesis.