中文摘要：

目的：探索前列腺Cajal间质细胞（interstitial cells of Cajal，ICCs）与交感神经及平滑肌细胞之间的形态及功能学联系。方法：（1）制作豚鼠前列腺组织冰冻切片并进行双重免疫荧光染色。使用酪氨酸激酶受体（c-Kit）作为ICCs的标记物，酪氨酸羟化酶（tyrosine hydroxylase，TH）和多巴胺β羟化酶（dopamineβ-hydroxylase，DβH）作为交感神经纤维的标记物，α肌动蛋白（α-actin）作为平滑肌细胞的标记物。另外，分别对α1-肾上腺素受体与c—Kit以及缝隙连接蛋白Connexin43（Cx43）与c—Kit进行免疫荧光双重标记。（2）将前列腺肌条固定于灌流槽中，记录去甲。肾上腺素（norepinephrine，NE）诱发的肌条收缩幅度和频率，以及加入Glivec后肌条收缩幅度和频率的变化。结果：（1）免疫荧光组织化学标记结果显示：ICCs与交感神经纤维、平滑肌细胞平行分布，ICCs边缘与交感神经、平滑肌细胞边缘密切接触，c-Kit标记的ICCs上存在α1-肾上腺素受体和缝隙连接蛋白Cx43的共表达。（2）在去甲肾上腺素的刺激下前列腺平滑肌肌条收缩幅度为（0．98±0．16）g，收缩频率为（2．54±0．28）次／min。使用Glivec后肌条收缩幅度显著下降，50μmol／L组为（0．56±0．07）g（P〈0．01），200μmol／L组为（0．25±0．05）g（P〈0．01），收缩频率未见明显改变，50μmol／L组为（2．67±0．32）次／min（P〉0．05），200μmol/L组为（2．75±0．31）次／min（P〉0．05）。结论：豚鼠前列腺ICCs具有介导前列腺交感神经信号，支配平滑肌活动的形态和功能学基础。

英文摘要：

Objective： To investigate the morphological and functional relationships of interstitial cells of Cajal （ICCs） with sympathetic nerves and smooth muscle cells in the guinea pig prostate. Methods ： （ 1 ） The guinea pig prostate slices were made for double-labeling immuno fluorescence histochemistry, e-Kit was used to distinguish ICCs, tyrosine hydroxy- lase （TH） and dopamine β-hydroxylase （DβH） were used to mark sympathetic nerve fibers, and the smooth muscle cells were labeled by α-actin. Gap junction protein connexin 43 （ Cx43 ） and α1-adrenoceptor were respectively double-labeled with c-Kit immuno histochemistry. （2） The prostate strips of guinea pig were mounted in a superfused organ-bath apparatus and the amplitude, frequency of norepinephrine （NE） -evoked smooth muscle contraction were recorded before and afterthe presence of Glivec. Results： （ 1 ） Immunofluorescence histochemical results showed that ICCs surrounded or were ar- ranged in parallel to sympathetic nerve bundles, the margin of ICCs made the close contact with sympathetic nerve fibers or smooth muscle cells. Cx43 and α1-adrenoceptor were coexpressed in prostate ICCs. （2） The amplitude of NE-evoked contraction of prostate strips was 0.98 ± 0. 16 g and the frequency was 2.54 ± 0.28/min. The amplitude were 0.56 ± 0.07 g and 0.25 ±0. 05 g after application of 50 μmol/L and 200 μmol/L Glivec, respectively （ P 〈 0.01 ）. The fre- quency were 2.67± 0.32/min and 2.75±0.31/min after application of 50μmol/L and 200 μmol/L Glivec, respectively （P 〉 0. 05 ）. Conclusion： ICCs in the guinea pig prostate possess the morphological and functional foundation which in- volved in mediating sympathetic signals and regulating the activity of smooth muscle.