中文摘要：

为探讨豚鼠膀胱Cajal样间质细胞（Interstitial cells of Cajal-like,ICCs）的原代培养及鉴定方法。方法：采用胶原酶消化法,得到原代培养的成年豚鼠膀胱ICCs细胞,应用免疫荧光法完成对ICCs的细胞鉴定。采用膜片钳技术,在全细胞记录模式下,记录正常对照组（体外培养72 h,葡萄糖浓度为5 mmol/L;72 h,G 5 mmol/L）、Ih电流鉴定组（体外培养72 h,葡萄糖浓度为5 mmol/L,检测时使用含CsCl 2 mmol/L的细胞外液;72 h,G 5 mmol/L,CsCl2 mmol/L）中ICCs细胞超极化激活时的Ih电流（hyperpolarization activated inward current,Ih）值。结果显示,正常对照组Ih电流数值（505.75±157.66）PA,Ih电流鉴定组（149.89±62.84）PA,正常对照组与高糖Ⅰ组比较P〈0.01。由此可知,用酶消化法可获得豚鼠膀胱Cajal间质细胞,在体外高糖环境培养基中生长良好并能检测出Ih电流。

英文摘要：

Objective：To explore the primary cultivation and identification method of the adult guinea pig bladder＇s ICCs in the high concentrations of glucose environment.Methods：Primarily cultured bladder ICCs of adult guinea pigs were harvested by collagenase digestion.By using immunofluorescence method,cell identification was made.We employed the whole-cell patch clamp technique to record the Ih current values of the ICCs in normal control group and Ih current identification group.Results： The Ih current value was 505.75±157.66 PA in the Nnormal control group,149.89 ＋ 62.84 pA in Ih current identification group（P0.01）.Conclusion：The enzymatic digestion is available to cultivate ICCs in guinea pig bladder,the cells grow well in the high concentrations of glucose medium,and Ih current can be detected.