中文摘要：

以豆科植物百脉根的mRNA为模板，利用RT—PCR方法，扩增到1．0kb结瘤因子受体5（nod factor receptor NFR5）蛋白激酶结构域的DNA片段（nfr5-pk），并将该片段克隆到酵母双杂交BD载体pGBKT7上。通过酵母双杂交技术，筛选接种根瘤菌的百脉根AD-cDNA酵母双杂交文库，从文库中分离到与nfr5-pk相互作用的221个阳性克隆。从这些阳性克隆酵母中分离AD质粒经反复验证，对26个确定阳性克隆的外源片段进行测序和同源性分析。通过NCBI数据库比对分析鉴定出小G蛋白Rop6等6个与NFR5-PK相互作用的不同蛋白，为进一步研究NFR5基因的功能和调控机制提供了材料。

英文摘要：

NFR5 is involved in signal perception and transduction of Nod factor produced by rhizobia in Lotus japonicus. In this research, by using yeast two hybrid approach, AD-cDNA library of L. japonicus was constructed and 221 positive clones were idendified by using the protein kinase domain of NFR5 （nfr5- pk） as a bait at pre-screening. After retranformed and X-Gal assay, 26 positive clones were confirmed. Six kinds of proteins including small G-Protein Rop6 were obtained after sequencing and blast search. These proteins proposed to be participated in function regulation NFRS.