中文摘要：

目的：探讨Hedgehog信号通路特异性抑制剂cyclopamine对人正常角质形成细胞HaCaT细胞白介素（IL）-8分泌的影响。方法：在HaCaT细胞培养基中加入cyclopamine，采用反转录（RT）-PCR和ELISA方法检测HaCaT细胞在cyclopamine处理后不同时段IL-8 mRNA及其分泌的变化。结果：HaCaT细胞在cyclopamine处理后IL-8的mRNA表达及其分泌均减少（P〈0．05）。结论：Hedgehog信号通路特异性抑制剂cyclopamine可抑制HaCaT细胞分泌IL-8。

英文摘要：

Objective： To investigate the influence of cyclopamine, a specific inhibitor of Hedgehog pathway, on the secretion of interleukin 8 （IL-8） in HaCaT cells. Methods： RT-PCR and ELISA were employed to measure the secretion of IL-8 in HaCaT cells at different time after treatment with cyclopamine. Results： IL-8 mRNA expression was significantly inhibited in HaCaT cells treated with cyclopamine, and IL-8 secretion was also reduced （P 〈 0.05）. Conclusion： Cyclopamine, a specific inhibitor of Hedgehog pathway, can suppress the IL-8 secretion of HaCaT keratinocytes.