中文摘要：

目的：探讨高血压状态下血管组织前纤维蛋白-1（Profilin-1）-信号转导和转录激活因子-3（STAT3）信号改变以及厄贝沙坦干预对该信号通路与氧化应激水平的影响。方法：采用10周龄C57/B6小鼠,经血管紧张素II（Ang II）诱导为高血压,分为高血压组（8只,安慰剂治疗）和厄贝沙坦组（8只,每天厄贝沙坦50mg/kg治疗）,另选正常血压小鼠（8只）为正常对照组。测量两周后血压水平,以Western印迹检测小鼠主动脉组织中Profilin-1表达及STAT3磷酸化水平,硫代巴比妥酸比色法测定丙二醛（MDA）含量。结果：与正常对照组相比,高血压组小鼠血压水平明显升高[收缩压：（104±4.4）mmHg比（159±6.2）mmHg],主动脉组织中Profi-lin-1蛋白表达[（1.00±0.08）比（2.91±0.12）]、STAT3磷酸化[（1.00±0.09）比（1.95±0.11）]以及MDA[（72.8±8.5）nmol/g protein比（165.2±13.6）nmol/g protein]水平明显增加（P均〈0.01）,而厄贝沙坦组血压明显下降[收缩压：（130±4.8）mmHg,P〈0.01],主动脉组织Profilin-1表达（1.53±0.07）和STAT3磷酸化（1.27±0.08）水平显著降低,伴有MDA含量显著下降[（103.7±10.1）nmol/g protein],P均〈0.05。结论：高血压状态下存在血管Profilin-1-STAT3信号上调,伴有氧化应激增强,而厄贝沙坦干预降低高血压小鼠血管Profilin-1表达及STAT3磷酸化水平,促使血管氧化应激水平改善,可降低血压。

英文摘要：

Objective：To investigate changes of vascular tissue Profilin-1-signal transducer and activator of transcription 3（STAT3）signaling and influence of irbesartan on it and oxidative stress level in hypertension.Methods： The 10-week C57/B6mice were infused with angiotensin II to establish hypertensive animal model.They were divided into hypertension group（n=8,received placebo treatment）and irbesartan group（n=8,received irbesartan 50mg/kg everyday）.Another eight mice with normal hypertension were regard as normal control group.Blood pressure was measured after two weeks;Western blotting method was used to measure levels of Profilin-1expression and STAT3phosphorylation in mice aortic tissue;thiobarbituric acid colorimetry was used to measure concentration of malonyl diadehyde（MDA）.Results：Compared with normal control group,there were significant increase in blood pressure[systolic blood pressure（SBP）：（104±4.4）mmHg vs.（159±6.2）mmHg],Profilin-1 protein expression in aortic tissue [（1.00±0.08） vs.（2.91±0.12）],STAT3phosphorylation [（1.00±0.09） vs.（1.95±0.11）] and MDA [（72.8±8.5） nmol/g pr otein vs.（165.2±13.6） nmol/g p rotein ] in hypertension group,P0.01all.After irbesartan treatment,there were significant decrease in blood pressure [ SBP ：（130±4.8） mmHg,P0.01 ],Profilin-1expression in aortic tissue（1.53±0.07）,STAT3phosphorylation（1.27±0.08） and MDA concentration [（103.7±10.1） nmol/g p rotein ] in irbesartan group,P0.05all.Conclusion：There is upregulated vascular Profilin-1-STAT3signaling and enhanced oxidative stress in hypertensive mice.Irbesartan treatment reduces vascular expression of Profilin-1and level of STAT3phosphorylation in hypertensive mice,contributing to improve vascular oxidative stress,and reduce blood pressure.