中文摘要：

瞄准：学习是否 H pylori 与长期的胆汁在病人的胆囊粘膜定位。方法：用 Warthy 满天星斗(W-S ) 银污点和免疫组织化学与 anti-H pylori 抗体染色，我们在胆汁的 524 种情况中屏蔽了石蜡标本。H pylori 脲酶基因 A (HPUA ) 和 H pylori 脲酶基因 B (HPUB ) 被聚合酶链反应(PCR ) 从胆汁的 81 个盒子在新鲜织物标本分析。结果：H 象 pylori 一样细菌用 W-S 污点在胆汁病人的 13.55% 胆囊被发现。同时，为 H pylori 抗体积极的细菌被免疫组织化学也与胆汁在病人的 7.1% 胆囊发现。81 个胆囊， 11 为 HPUA 和 HPUB 是积极的， 4 仅仅为 HPUA 是积极的， 7 仅仅为 HPUB 是积极的。结论：H pylori 与长期的胆汁在病人的胆囊存在。

英文摘要：

AIM： To study whether Hpylori locate in the gallbladder mucosa of patients with chronic cholecystitis. METHODS： Using Warthy-Starry （W-S） silver stain and immunohistochemistry stain with anti-H pylori antibodies, we screened paraffin specimens in 524 cases of cholecystitis. Hpylori urease gene A （HPUA） and Hpylori urease gene B （HPUB） were analyzed by polymerase chain reaction （PCR） in the fresh tissue specimens from 81 cases of cholecystitis. RESULTS： H pylori-like bacteria were found in 13.55% of the gallbladders of the cholecystitis patients using W-S stain. Meanwhile, bacteria positive for H pylori antibodies were also found in 7.1% of the gallbladders of patients with cholecystitis by immunohistochemistry. Of 81 gallbladders, 11 were positive for both HPUA and HPUB, 4 were positive for HPUA only and 7 were positive for HPUB only. CONCLUSION： Hpylori exist in the gallbladders of patients with chronic cholecystitis.