中文摘要：

瞄准：学习 Helicobacter pylori (H pylori ) 由损坏人的胆囊上皮细胞(HGBEC ) 的机制。方法：从胆囊孤立的 H pylori 在液体媒介是有教养的。不同集中上层清液和 H pylori 房间的声音的 ated 摘录然后在一种主要文化被加到 HGBEC。在象在硷性磷酸酯酶(高山) 的层次的变化一样的 HGBEC 的词法变化， lactate 脱氢酶(LDH ) 和 glutamyltransferase (GGT ) 被测量。结果：根据 HGBEC 的文化曲线，由增加声音的 ated 提取的 H pylori 和 H pylori 文化上层清液在 HGBEC 学习变化是方便的。声音的 ated 提取的 H pylori 和 H pylori 文化上层清液在 HGBEC 形态学上有重要影响，即 HGBEC 更慢慢地成长了，他们的生存能力减少了，他们的分开增加了。而且， HGBEC 破裂了并且死。高山的层次( 33.84 +/- 6.00 对 27.01 +/- 4.67 ， P 【 0.05 )， LDH ( 168.37 +/- 20.84 对 55.51 +/- 17.17 ， P 【 0.01 )并且 GGT ( 42.01 +/- 6.18 对 25.34 +/- 4.33 ， P 【 0.01 )显著地在一个时间依赖者和集中依赖者在 HGBEC 文化上层清液增加了。在 H pylori 文化液体的 HGBEC 的损坏比在 H pylori，声音的 ated 提取是更重要的。结论：H pylori 不导致明显的损坏到 HGBEC。

英文摘要：

AIM： To study the mechanism by which Helicobacter pylori （H pylori） damages human gallbladder epithelial cells （HGBEC）. METHODS： H pylori isolated from gallbladder were cultured in a liquid medium. Different concentration supernatants and sonicated extracts of H pylori cells were then added to HGBEC in a primary culture. The morphological changes in HGBEC as well as changes in the levels of alkaline phosphatase （ALP）, lactate dehydrogenase （LDH） and glutamyltransferase （GGT） were measured. RESULTS： According to the culture curve of HGBEC, it was convenient to study the changes in HGBEC by adding H pylori sonicated extracts and H pylori culture supernatants. Both H pylori sonicated extracts and Hpylori culture supernatants had a significant influence on HGBEC morphology, i.e. HGBEC grew more slowly, their viability decreased and their detachment increased. Furthermore, HGBEC ruptured and died. The levels of ALP （33.84 ± 6.00 vs 27.01 ± 4.67, P 〈 0.05）, LDH （168.37 ± 20.84 vs 55.51 ± 17.17, P 〈 0.01） and GGT （42.01 ± 6.18 vs 25.34 ±4.33, P 〈 0.01） significantly increased in the HGBEC culture supernatant in a time- and concentrationdependent. The damage to HGBEC in H pylori culture liquid was more significant than that in Hpylori sonicated extracts. CONCLUSION： H pylori induces no obvious damage to HGBEC.