中文摘要：

目的：观察前列腺癌组织RECK基因及基质金属蛋白酶-9（MMP-9）的表达，探讨前列腺癌发生中RECK基因的可能作用机制。方法：取20例前列腺癌及12例正常前列腺组织，RT—PCR及real—time RT—PCR检测RECK基因表达，RT—PCR法检测MMP-9的表达，Western印迹法检测RECK蛋白表达。结果：RT—PCR及real—time RT—PCR检测发现前列腺癌组织RECK基因表达明显低于正常组织（P〈0．01），而MMP-9mRNA表达明显高于正常组织（P〈0．01）。Western印迹分析见前列腺癌组织中RECK蛋白的表达明显低于正常组织（P〈0．01）。结论：前列腺癌组织中RECK基因表达较低，RECK基因可能通过抑制前列腺组织MMP-9表达发挥抑癌作用。

英文摘要：

Objective： To investigate the expression of the reversion-inducing cysteine-rich protein with Kazal motifs （RECK） and matrix metalloproteinase-9（MMP-9） in the prostate carcinoma tissues and to evaluate the role of RECK in the tumorigenesis of prostate carcinoma. Methods：Twenty specimens of prostate cancer and 12 specimens of normal prostate were harvested. RT-PCR and real-time RT-PCR were used to determine the expression of RECK mRNA and RT-PCR was used to determine the expression of MMP-9 mRNA in the specimens. Western blotting analysis was used to determine the expression of RECK protein. Results： It was found that the expression of RECK mRNA in the prostate carcinoma tissues was lower than that in the normal prostate tissues （P〈0. 01 ）; MMP-9 expression in the prostate carcinoma tissues was significantly higher than that of the normal prostate tissues （P〈0.01）. Western blotting analysis showed that the expression of RECK protein in the carcinoma tissues was lower than that in the normal prostate tissue （P〈0.01）. Conclusion： RECK gene expression is lower in the prostate carcinoma tissues; RECK may inhibit the progression and metastasis of cancer through inhibiting MMP-9 expression.