中文摘要：

目的 检测RECK基因在不同前列腺细胞株BPH-1、DU-145、LNCap以及PC-3中的表达,探讨其表达差异及其与基质金属蛋白酶2（MMP-2）的关系.方法 应用RT-PCR技术及Real-time PCR技术检测4种不同前列腺细胞株中RECK基因及MMP-2 mRNA的表达;应用Western blot检测不同前列腺细胞株中RECK基因的表达.结果 前列腺癌细胞株DU-145、LNCap以及PC-3中RECK mRNA的表达较良性前列腺增生细胞株BPH-1中明显降低（P＜0.01）,MMP-2 mRNA表达则明显升高.DU-145、LNCap以及PC-3细胞株中RECK基因的表达较正常组织明显降低.结论 RECK基因在前列腺癌中的表达量明显下降,而MMP-2的表达量明显升高,RECK基因可能通过抑制MMP-2的表达起到抑癌基因的作用.

英文摘要：

Objective To investigate the role of PECK gene in the progression and invasion in the prostate carcinoma. Methods The mRNA level was measured by RT-PCR and real-time PCR, the protein level was evaluated by Western blot. Results The mRNA level of the PECK gene on the prostate carcinoma cell strains （DU-45, LNCap and PC-3） was lower than that on the benign prostate hyperplasia cell strain BPH-1 ,while the matrix metalloproteinase-2 （MMP-2） level was higher. Also the protein level of RECK on the DU-45, LNCap and PC-3 was lower than that on the BPH-1. Conclusion PECK gene was supposed to be a kind of tumor suppression gene, which may act by inhibiting the activity of MMPs.