中文摘要：

为了提高生防菌株莫海威芽孢杆菌（Bacillus mojavensis ZA1）液体发酵的生物量,利用单因素试验设计与响应曲面试验设计相结合的方法对ZA1 摇瓶发酵条件进行了优化.结果表明,ZA1 的最佳培养基配比为氯化铵14.25g、玉米粉19g、马铃薯237g、水1000mL,最佳发酵条件为pH7.7、培养温度28℃、转速180r/min及发酵时间36h,ZA1优化后活菌数为4.12×10^10 CFU/mL.通过中心组合试验设计确定ZA1在10L 发酵罐中的最佳溶氧量为60%和转速为180r/min;最优条件下,发酵ZA1 的放罐时间确定为36h,活菌数达到1.59×10^11 CFU/mL.该结果为利用ZA1开发生防制剂奠定了基础.

英文摘要：

This work aimed to identify optimum fermentation shaker-flask culture conditions for Bacillus mo-javensis strain ZA1.Various single culture factors were combined according to a Box-Behnken design.The op-timum ratio of ingredients for the culture medium for ZA1was found to be:14.25 g ammonium chloride,19 g corn flour,and 237 g potato,in 1000 mL distilled water.The optimum cultivation conditions were pH 7.7, temperature 28℃,and shaken at 180 r/min for 36 h.Under these conditions,the viable count of B .mojavens-is strain ZA1 was 4.12×10 10 colony-forming units per mL (CFU/mL).Further testing using the central com-posite design method in a 10 L fermentation tank found the optimum for dissolved oxygen level and shaker rota-tion speed to be 60% saturation and 180 r/min respectively.Under these conditions,when fermentation time was 36 h in the fermentation tank,the viable count of B .mojavensis strain ZA1 reached 1.59×10 11 CFU/mL. The results of this study lay a solid foundation for the development of methodology for large scale production of biocontrol agents.