中文摘要：

为建立尾巨桉高效再生体系，研究基本培养基对尾巨桉再生的影响，以尾巨桉优良无性系无菌苗茎段为外植体，探讨MS、SDM和SPM3种基本培养对尾巨桉愈伤组织诱导、不定芽分化以及增殖的影响。结果表明，在添加T2mg／LPBU和110．05mg／LIAA的MS培养基上，茎段外植体5d后愈伤组织诱导率达96．3％以上；将愈伤组织接种在添加1mg／L6-BA和0．05mg／LNAA的MS培养基上，诱芽率达91．8％，不同基本培养基对不定芽的增殖及伸长的影响差异达到极显著水平。

英文摘要：

The callus induction and shoot regeneration were studied systematically in elite clone of Eucalyptus urophylla x E. grandis by comparing the basal mediums. The results showed that sterile clones of seedling stem segments were used as explants and cultured in a MS medium supplemented with the synthetic growth regulator 2 mg/L PBU and 0.05 mg/L IAA. After cultivation for 5 d, 96.3% of explants formed callus. Callus were transferred to MS medium containing 1 mg/L 6-BA and 0.05 mg/L NAA to induce bud formation, and 91.8% of the callus induced by PBU exhibited adventitious bud formation. Effect of basic mediums on the adventitious shoot proliferation and development from hypocotyls were greatly significant differences.