中文摘要：

目的探讨电针治疗阿尔茨海默病的可能作用机制。方法将快速老化小鼠P8亚系（SAMP8）36只随机分为模型组、电针组和药物组，抗快速老化小鼠R1亚系（SAMR1）12只为正常组。电针组给予电针百会穴、印堂穴20min后，点刺水沟穴；药物组给予盐酸多奈哌齐按0．92mg·kg-1剂量灌胃；正常组和模型组给予电针组相同程度和时间的束缚。治疗15d后，用Morris水迷宫测定小鼠学习记忆能力，免疫组化技术检测小鼠大脑皮层B淀粉样蛋白（Ap）及β位淀粉样前体蛋白裂解酶（BACE-1）含量，实时荧光定量PCR法测定BACE-1mRNA的表达。结果与模型组比较，药物组与电针组逃避潜伏期均显著下降，而目标象限游泳时间均显著增加（P〈0．05），电针组BACE-1、BACE-1mRNA表达显著减少（P〈0．05）；电针组游泳总路程、游泳速度明显下降，与药物组比较差异有统计学意义（P〈0．05）。结论电针可能通过抑制SAMP8小鼠大脑皮层BACE-1表达，降低AB水平，达到改善其学习记忆障碍的作用。

英文摘要：

Objective To discuss the potential effect mechanism on Alzheimer~s disease with dec- troacupuncture. Methods Thirty - six mice of senescence - accelerated mice prone 8 （ SAMP8 ） were ran- domized into a model group, an electroacupuncture group and a medicine group. Twelve mice of senescence accelerated mouse/resistance 1 （ SAMR1 ） were in a normal group. In the electroacpuncture group, electroacu- puncture was applied to Baihui （GV20） and Yintang （ EX - HN3 ） for 20min. Afterwards, the pricking tech- nique was used at Shuigou （GV26）. In the medicine group, the gastric infusion with Donepezil HC1, at 0. 92mg/kg was applied. In the normal group and the model group ,the same degree and time restriction were a- dopted as the electroacupuncture group. After treatment for 15 days, Morriz water maze test was used to deter- mine the learning and memory ability of mice. IHC technology was used to determine the contents of 13 amy- loid protein（Aβ）and β amyloid precursor protein cleaving enzyme （BACE -1 ）. The real -time fluorescent quantitative PCR was applied to determine the expression of BACE - 1 mRNA. Results Compared with the model group, the escaping latent period was reduced significantly in the medicine group and the electroacu- puncture group, and the target quadrant swimming time was increased significantly （ P 〈 0.05 ） ; the expres- sions of BACE - 1 and BACE - 1 mRNA were reduced significantly in the electroacupuncture group （ P 〈 0. 05 ）. The total distance and velocity of swimming were reduced apparently in the electroacupuncture, indica- ting the significant difference as compared with the medicine group （P 〈 0.05 ）. Conclusion Electroacu- puncture has the improvements in learning and memory disturbance probably through inhibiting BACE - 1 ex- pression and reducing Aβ level of cerebral cortex in SAMP8 mice.