中文摘要：

为有效利用带鱼加工下脚料类资源,本研究采用凝胶层析法对制备所得的带鱼加工下脚料酶解亚铁螯合物进行了分离分级,初步确定了不同分离组分的分子量及回收率,采用氨基酸自动分析仪确定了不同分子量组分的氨基酸成分与含量,并进行了氨基酸评分;而后通过缺铁性贫血大鼠模型研究了不同分子量组分对大鼠贫血的改善效果。结果显示,带鱼下脚料经酶解、亚铁螯合修饰和层析分离后得到4类分离产物,分子量分别约为35 400,8 720,465和小于200 u;除组分Ⅱ含有8种必需氨基酸外,其他组分均属于不完全蛋白质;理想氨基酸模式评价结果表明4类螯合物氨基酸价分别达到理想氨基酸模式的85.31%、88.22%、101.32%和105.83%,仍然属于质量较高的蛋白质;不同分子量螯合物中铁含量存在显著差异,其中以组分Ⅰ含量最高（5.23%）,组分Ⅱ含量最低（3.34%）,组分Ⅲ和组分IV铁含量接近（4.22%和4.58%）;不同分子量螯合物对大鼠体质量、Hb、血清铁、血清铁结合力、肝脏M DA和SOD影响不同,其中组分Ⅱ对缺铁性贫血大鼠贫血改善效果最好,且效果优于Fe SO4,组分Ⅰ在螯合物中效果最差,但仍有一定改善效果。

英文摘要：

This paper focused on the high efficient utilization of by-product of Trichiurus haumela. The hydrolyzed ferrous chelate prepared from by-product of T. haumela was prepared and initially classified by the method of gel chromatography, and recovery and molecular weight （MW） were confirmed. The constitutes and content of amino acid of different chelates were investigated using automatic amino acid analyzer, while the amino acid score were also confirmed. The therapeutic effect of different molecular weight chelates in improving iron-deficiency anemia was conducted by IDA model rats. The results showed that the chelation was classified into 4 materials,the molecular weight of which were 35 400,8 720,465 and less than 200 u,respectively. All of these materials belonged to incomplete protein except component 11. The evaluation results of amino acid model of ideal protein showed that AAS of these 4 materials were 85.31% , 88.22% ,101.32% ,105.83% ,and were also high quality protein. Content of Fe differed significantly from each other,the content of Fe of component Ⅰ and Ⅱ were 5.23% and 3.34% ,and there was a similarity in Fe content between Ⅲ and Ⅳ （4.22% and 4.58% ）. The results of rat experiment showed that different chelation did not have the same effect on weight, serum iron,TIBC,liver MDA and SOD,and component Ⅱ showed the optimal effect in anemia improvement compared with FeSO4. Though component Ⅰ did not possess the significant effect,it also had a certain ability in curing anemia.