中文摘要：

通过反转录PCR法成功地扩增出日本曲霉β-呋喃果糖苷酶基因，基因片断长度为1917bp，编码638个氨基酸。将所得片断定向克隆到pYX212载体上，并转化至酿酒酵母中，通过酵母菌落PCR检验后，确定该基因在酿酒酵母中获得表达，转化子平均酶活为26．4U／mg。

英文摘要：

A β-fructofuranosidase gene of 1 917 bp was cloned from Aspergillus japonicus, and the ORF sequence predicted a protein of 638 amino acids. A expression plasmid pYX212 carrying the gene was under the control of the triose phosphate isomerase gene （TPI） promotor was transformed into Saccharomyces cerevisiae W303-1A, and β-fructofuranosidase gene was successfully expressed in the recombinant strains. The enzyme activity was about 26.4 U/mgo