利用Penicillium sp.ZD-Z1发酵培养生产壳聚糖酶,研究其降解特性,并制备特定低分子量的壳聚糖。结果表明,在30℃、pH5、加内切酶ChA 0.01u/ml的条件下降解4%壳聚糖,通过MHS方程77=1.02×10^-4Mw^1.227控制反应时间,可以得到低分子量的壳聚糖。
Chitosanase was prepared from Penicillium sp. ZD-Z1. The hydrolytic condition of chitosanase to produce chitosan with special molecular weight were examined. The results indicated that chitosan with low molecular weight could be obtained by hydrolyzing 4% chitosan with 0.01u/ml ChA chitosanase at 30℃, pH 5, using MHS equation η= 1.02 × 10^-4Mw^1.27 to control reaction time.