为研究亚油酸底物葵花籽油对山羊瘤胃消化代谢和瘤胃液脂肪酸组成的影响,以5只装有永久性瘤胃瘘管的雌性成年徐淮白山羊为试验动物,采用自身对照设计,对照期和试验期(添喂葵花籽油7 mL/d)各15 d,低精料日粮常规饲养。每期结束后采集瘤胃液进行分析。结果表明,添喂葵花籽油后,瘤胃液pH无显著变化;而氨氮(NH3-N)水平和TVFA显著升高(P〈0.05);乙、丙酸含量显著增加(P〈0.05),而乙、丙、丁酸比例(C2+C4)/C3比值和微生物粗蛋白(MCP)均无明显变化;(C18∶1)和c9,t11-CLA(P〈0.05)浓度均明显升高。表明在山羊日粮中添加一定量的葵花籽油,可在不改变瘤胃发酵类型和不影响瘤胃微生物本身的情况下促进瘤胃消化代谢,调节瘤胃液脂肪酸组成。
This experiment was conducted to evaluate effects of sunflower seed oil supplementation on rumen digestion and fatty acid composition. 5 female adult Xuhuai White goats were surgically installed with a ruminal fistula. The experiment was divided into two periods by self-control design. Each period lasted for 15 days. Goats were fed a low-concentrate diet in two periods (non sunflower seed oil or 7 mL sunflower seed oil per day). Rumen liquid was taken from the rumen fistula at the last day of each period. The results showed that the pH value of ruminal liquid was not affected by sunflower oil supplementation. Compared with control period, the concentration of ammonia N, total volatile fatty acid (TVFA), acetate and propionate in the oil supplementation period increased significantly (P〈0.05). However, the contents of acetate, propionate and butyrate, the ratio of acetate plus butyrate to propionate (C2 + C4)/C3, microbial crude protein (MCP) concentration in ruminal liquid were not affected by the oil supplementation. The concentration of oleic acid (C18 : 1) and cis9, trans11-conjugated linoleic acid (c9,t11- CLA) in ruminal fluid increased remarkably by sunflower oil supplementation compared with control period (P〈0. 05). The results implicated that appropriate dose of sunflower oil supplementation could improve rumen digestion and manipulate long chain fatty acid composition in goats fed low-concentrate diet.