中文摘要：

目的：观察肿瘤坏死因子-α（tumor necrosis factor-α,TNF-α）对人脐静脉内皮细胞（HUVECs）组织型纤溶酶原激活物（tissue plasminogen activitor,t-PA）及其抑制剂-1（plasminogen activitor inhibitor-1,PAI-1）表达的影响。方法：原代分离HUVECs细胞并进行传代培养,分别以6个TNF-α浓度组（0、1、10、20、50、100 ng·m L-1）处理不同时间（0、1、3、6、12、24 h）,酶联免疫吸附分析（ELISA）法测定t-PA、PAI-1抗原的表达;逆转-聚合酶链反应（RTPCR）检测t-PA、PAI-1基因的表达。结果：TNF-α促进PAI-1抗原的表达,并呈剂量和时间依赖关系,在TNF-α10 ng·m L-1作用6 h时最明显（P〈0.01）;TNF-α促进PAI-1 mRNA的表达,并呈剂量和时间依赖关系,在TNF-α10 ng·m L-1作用3 h时已非常明显（P〈0.01）,6 h时达到高峰（P〈0.01）;而TNF-α对HUVECs表达t-PA抗原、mRNA无明显影响。结论：炎症因子TNF-α可能通过上调PAI-1表达而诱发血栓相关疾病。

英文摘要：

Objective： To observe the effects of tumor necrosis factor-α（ TNF-α） on the tissue plasminogen activator（ t-PA） and plasminogen activitor inhibitor-1（ PAI-1） expression in human umbilical vein endothelial cells（ HUVEC）. Methods： Original HUVECs cell were isolated and subcultured. They were divided into six TNF-α concentration groups（ 0,1,10,20,50,100 ng·m L- 1） and were dealt with in different hours（ 0,1,3,6,12,24 h）. The expressions of T-PA and PAI-1 antigen were determined by ELISA; t-PA mRNA and PAI-1 mRNA were examined by reverse transcription polymerase chain reaction（ RT-PCR）. Results： TNF-α promoted PAI-1 antigen expression in HUVECs in a dose or time independent manner,reaching a maximum level with TNF-α 10 ng·m L- 1after 6 hours（ P 0. 01）; TNF-α promoted PAI-1mRNA expression in HUVECs in a dose or time independent manner,being fairly apparent with TNF-α 10 ng·m L- 1after 3 hours（ P 0. 01） and reaching a maximum level with TNF-α 10 ng·m L after 6 hours（ P 0. 01）; There were no effect on the t-PA antigen and mRNA exprssion induced by TNF-α in HUVECs. Conclusion： TNF-α may induce thrombosis related diseases by enhancing PAI-1 expression.