中文摘要：

目的研究三羟异黄酮（GST）对永久性大脑中动脉栓塞（pMCAO）模型大鼠的神经保护作用，及其对神经干细胞增生及神经再生相关因子含量的影响。方法将大鼠随机分为假手术组、对照组、GST治疗组，制作pM—CAO模型，治疗组于术后3h腹腔注射GST50、100μg/（kg·d），连续注射5d，对照组注射等量生理盐水。采用神经行为学评分、脑梗死体积测定、尼氏染色、BrdU—Nestin免疫荧光双标ELISA等方法，观察GST对大鼠神经损伤、功能恢复、脑内相关部位神经干细胞增生、神经再生相关因子表达水平的影响。结果与对照组相比，GST治疗组神经行为学评分明显提高，神经干细胞数目明显增多，神经再生相关因子含量明显升高（P〈0．05或〈0．01）。结论GST治疗能改善pMCAO大鼠神经行为学评分，减少脑梗死体积，进一步增加增生的神经干细胞数目及神经再生相关因子的含量，对脑组织具有神经保护作用。

英文摘要：

Objective To investigate the neuroprotective effect of genistein （GST） and its influences on the prolifera- tion of neural stem cells and the expression of neuroregeneration-related factors in brain tissue of rats with permanent middle cerebral artery occlusion （pMCAO）. Methods Adult male SD rats were subjected to permanent middle cerebral artery oc- clusion （pMCAO） and randomly divided into the sham-operation group, control group and GST-treated group. GST was in- traperitoneally injected for 5 days at a dose of 50 or 100μg/（ kg · d） , 3 h after pMCAO. The control group was injected with the same dose of normal saline. TTC staining, Nissl staining, BrdU/nestin immunofluorescent double staining of neu- ral stem cells （NSCs） and ELISA methods were conducted to determine the influence of GST on the neurological function, the proliferation of NSCs, and the expression of neuroregeneration-related factors in brain tissue. Results Compared with the control group, the neurological behavior score, the number of BrdU/nestin-positive cells and the expression level of neuroregeneration-related factors in the brain tissue after pMCAO in the GST-treated group were much higher （ P 〈 0.05, or 〈 0.01 ）. Conclusions GST can improve the neurological behavior score, reduce the infarct volume and promote the pro- liferation of the endogenous NSCs and the expression of neuroregeneration-related factors in the brain tissue of pMCAO rats, meanwhile it has neuroprotective effect on the brain tissue.