通过施用有机肥及增加外源铜的投入,研究了有机肥对土壤中铜的形态及其生物效应的影响。结果表明,增加外源铜的投入和施用有机肥都会改变铜在不同形态间的分配,随外源铜投入量的增加,交换态组分增加的幅度较小,其他形态则增加幅度较大,其中有机结合态、锰结合态的相对比例增加迅速。在200 mg/kg铜处理中,含量分别为无铜对照的28.3倍和25.8倍,占总量的比例分别由17.3%和15.9%上升为39.6%和33.3%。随着有机肥用量的增加,锰结合态组分含量相对降低,在无外源铜投入时其含量在0%和6%有机肥用量下分别为1.77,1.61 mg/kg;当外源铜投入量为200 mg/kg时,0%和6%有机肥处理的锰结合态组分分别为61.48,26.04 mg/kg。有机结合态组分则随有机肥的用量增加而增加,在无外源铜投入时,其含量在0%和6%有机肥水平下分别为1.37,2.45 mg/kg;在外源铜加入量为200 mg/kg时,0%和6%有机肥水平下有机结合态铜分别为25.83,71.83 mg/kg。草莓根系铜含量与各形态铜皆有极显著正相关关系,而地上部铜含量仅与有机结合态铜无显著相关关系,有机肥处理对草莓铜含量也无显著影响。
Influence of manure application and copper input on copper fractionation and its biological effects were studied in this paper.The results showed that manure application and copper input all could affect the distribution of copper among different fractions.With the increase of copper input,the exchangeable copper only had a slight increase,while the other fractions increased rapidly.The relative amount of organically bound and manganese oxide bounded copper increased more rapidly.In the 200 mg/kg copper treatment,they were 28.3 times and 25.8 times that of the control,their percent in the total copper increased from 17.3% and 15.9% to 39.6% and 33.3% respectively.With manure application,the manganese oxide bounded fraction decreased apparently.In no-copper input soil,the amount of manganese oxide bounded fraction decreased from 1.77 mg/kg of the control to 1.61 mg/kg of the 6% manure treatment.In the 200 mg/kg copper input soil,manganese oxide bounded copper decreased from 61.48 mg/kg of the control to 26.04 mg/kg of the 6% manure treatment.The organically bounded fraction increased with manure application.In soil without external copper input,the organically bounded copper increased from 1.37 mg/kg of the control to 2.45 mg/kg of the 6% manure treatment.When the copper input was 200 mg/kg,the organically bounded copper fractions in control and 6% manure input treatment were 25.83,71.83 mg/kg respectively.Significant positive correlation of strawberry copper content with all soil copper fractions was found except that of organically bounded part with above ground copper.Manure application had little effects on strawberry copper in this experiment.