中文摘要：

Pm4是我国目前有效的小麦抗白粉病基因之一,该基因对陕西关中当前流行的白粉病菌系关中4号表现高抗。本研究利用与Pm4基因紧密连锁的STS470分子标记对7个小麦品种间的6个杂交组合F3代单株进行分子标记检测并结合田间抗性鉴定筛选出抗病单株14个。对由这14个单株衍生出的64个F5代品系进行分子检测和田间抗性鉴定,发现53个品系表现抗病,占总品系的82.81%,其中51个抗病品系可以检测到与Pm4基因紧密连锁的STS470特异带,占总品系的79.69%。本研究结果表明,STS470是Pm4基因的可靠标记,可有效的用于分子标记辅助育种。

英文摘要：

Pm4,a high resistant gene to the prevalent race Guanzhong 4 of powdery mildew pathogen,is one of the most effective wheat powdery mildew resistant gene in China.Molecular marker assistant selection is not limited by environment and cropping season,its application in the breeding program can improvement the accuracy of selection and breeding efficiency.In this study,14 disease resistant F3 plants from six crosses of seven parents were selected by both STS470 marker and field disease test.Sixty four F3 lines,derived from the 14 F3 plants,were tested in the field and screened by molecular marker STS470.Among the 64 lines,there were 53 lines（82.8%） showed high resistance to the powdery mildew,of which 51 lines（79.7%） with resistant molecular marker STS470.The results showed that STS470 was a reliable molecular marker for screening Pm4 resistant gene and it could be effectively used in wheat breeding.