中文摘要：

聚丙烯酰胺凝胶（PAGE）电泳是一种常用的DNA检测方法，广泛应用于现代生物技术研究。PAGE电泳中的银染技术复杂，银染过程中所要用到的聚丙烯酰胺、过硫酸铵、硝酸银等几种药品的浓度和作用时间直接影响实验效果。6％的凝胶分辨率较高；0．2％比0．1％硝酸银显带灵敏度高；甲醛浓度在0．1％～0．2％（体积比）范围内显色差异不大；1．5％和3％氢氧化钠显色结果无差别；10mg／mL的硫代硫酸钠用量应为100～200μL／L；弱碱、强碱和改良银染法各有其优缺点，研究者可根据研究需求选择适合的银染方法。弱碱法染色背景浅，适于品种指纹图谱分析。强碱法及简易银染法具有高效率、低成本和操作简便的特点，适用于分子标记辅助育种。

英文摘要：

Silver staining affected by many factors is a complex technology in the polyacrylamide gel electrophoresis. The concentration and work time of polyacrylamide, ammonium persulfate, silver nitrate etc which are used in silver staining have direct impact to experimental results. 6% gel has high-resolution. 0.2% silver nitrate is more sensitive than 0. 1%. Concentration of formaldehyde at 0.1% -0.2% （by volume） has no difference in color. 1.5% and 3% sodium hydroxide has no difference in color results. Dosage of 10 mg/mL of sodium thiosulfate should be 100 -200 μL/L. Weak base, strong base and improved silver staining methods have their advantages and disadvantages. Researchers can choose appropriate silver staining method according to the needs of research. Weak base staining background light is suitable for variety of fingerprint analysis. Strong base and simple silver staining method with high efficiency, low cost, simple operation, is suitable for marker-assisted breeding.