利用抗、感青枯病的花生品种为亲本配制杂交组合中花5号×远杂9102,构建重组近交系,以其F6为研究材料,分析青枯病抗性遗传规律,结果表明,花生青枯病抗性是由两对主效基因控制的遗传,并且主效基因的遗传力较高,为84%;同时采用AFLP技术和BSA分析方法,获得两个与花生青枯病抗性连锁的分子标记,标记与抗性间的遗传距离分别为8.12cM和11.46cM。利用获得的分子标记对抗、感青枯病的花生种质进行了分子鉴定,证实了标记P3M59与青枯病抗性的符合率为70%,标记P1M58的符合率为50%,从而为花生青枯病抗性辅助选择育种提供理论基础。
The F6 of a peanut RIL population derived from the combination of Zhonghua 5 (susceptible)× Yuanza 9102 (resistance) were used to analyze the inheritance of resistance to bacterial wilt. The result revealed that inheritance of the resistance is controlled by two major genes with high heritability (84%). Two DNA markers, named P3M59 and P1M5, linked with the resistance (8.12 cM and 11.46 cM respectively) were detected using AFLP technique combined with bulked segregant analysis. Ten peanut genotypes with resistance to bacterial wilt and three susceptible genotypes were used to verify the reliability of the resistance markers, and high correlation was detected (70% and 50% respectively). The results showed that these markers can be used in marker assistant selection in peanut breeding.