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利用SSR技术研究花生属种间亲缘关系
  • ISSN号:1672-1810
  • 期刊名称:《植物遗传资源学报》
  • 时间:0
  • 分类:Q949.718.2[生物学—植物学]
  • 作者机构:[1]中国农业科学院油料作物研究所,武汉430062
  • 相关基金:国家自然科学基金(30270840;30571132)
中文摘要:

用44对SSR特异引物对花生属不同区组的21份种质进行了分析,获得能稳定揭示花生属种间差异的SSR引物34对。34对引物在21份种质中共检测到190个等位基因变异,每个位点上检测的等位变异数为3~11个,平均为5.59个。21份材料间存在很大的遗传变异,平均遗传距离为0.63,变异范围为0.08~0.95。聚类分析表明,区组间的遗传分化大于区组内的遗传分化,匍匐区组的遗传分化大于其他区组的遗传分化,同一物种不同种质问也存在很大的差异。栽培种花生与花生区组材料的亲缘关系相对较近,与花生属的植物学分类一致,其中A基因组的A.duranensis和A.villosa及B基因组的A.batizocoi与栽培种花生的亲缘关系更近一些。

英文摘要:

Forty-four SSR primer pairs were used to amplify genomic DNA of 21 peanut genotypes in genus Arachis. Thirty-four of the 44 primers were detected which displayed polymorphism among 21 peanut genotypes and amplified 190 loci alleles in total. The average number of alleles per locus was 5.59 with range of 3 to 11. The genetic diversity level among 21 genotypes in genus Arachis were very high and genetic distance (GD) was from 0.08 to 0.95 with an average of 0.63. The results from UPGMA analysis indicated that genetic differentiation among the genotypes from different sections was bigger than that from the same section. Genetic diversities of species in section Procumbentes was richer than that in other sections. There was also considerable polymorphism among the different genotypes of one same species. The genetic distance between cultivated peanut and several species including A. duranensis, A. villosa and A. batizocoi belonging to section Arachis was relatively more close in the comparison with some other species which was in accordance with peanut classification system.

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期刊信息
  • 《植物遗传资源学报》
  • 北大核心期刊(2011版)
  • 主管单位:中华人民共和国农业部
  • 主办单位:中国农业科学院作物科学研究所 中国农学会
  • 主编:刘旭
  • 地址:北京中关村南大街12号
  • 邮编:100081
  • 邮箱:zwyczyxb2003@163.com/sina.com/caas.cn
  • 电话:010-82105794 82105795
  • 国际标准刊号:ISSN:1672-1810
  • 国内统一刊号:ISSN:11-4996/S
  • 邮发代号:82-643
  • 获奖情况:
  • 国内外数据库收录:
  • 美国化学文摘(网络版),中国中国科技核心期刊,中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:10278