中文摘要：

目的：建立兴仁金线莲HPLC指纹图谱。方法：采用Phenomenex C18（4.6mm×250mm,5μm）;流动相：乙腈（A）-0.4%磷酸水溶液（B）梯度洗脱程;流速：1.0ml/min;检测波长：327nm;柱温：30℃。运用中药色谱指纹图谱相似度评价系统软件（2004A版）进行指纹图谱分析。结果：分别优化了检测波长、流动相以及HPLC色谱梯度洗脱条件,标定了20个共有指纹特征峰,确定了以槲皮素、山柰素和异鼠李素3个化合物,建立了12个不同批次的兴仁金线莲HPLC指纹图谱,相似度较高。结论：该方法方法学考察符合指纹图谱技术要求,为兴仁金线莲质量控制提供依据。

英文摘要：

Objective To establish a HPLC fingerprint of Anoectochilus xingrensis. Methods The chromatographic separation was performed on a Phenomenex C ls column （4. 6 × 250mm, 5 μm）, mobile phase was acetonitrile -0. 4% phosphoric acid. and the sepa- ration process was carried out using a gradient elution, the corresponding concentration gradient of acetonitrilewhich was based on a mo- bile phase of the mixture solution with acetonitrile （A） - 0.4% phosphoric acid （B） at the flow rate of 1. 0 mL/min. The detection wavelength was 327nm, and the column temperature was 30℃. The chromatographic fingerprints were analyzed with the similarity eval- uation system for chromatographic fingerprint of TCM （2004A edition）. Results Totally 20 common fingerprint peaks and 3 constituents including quercetin, kaempferide and isorhamnetin were identified after optimization of the detection wavelength, mobile phase and HPLC chromatography gradient elution conditions. Furthermore, the similarity of HPLC fingerprints of 12 different batches of Anoec- tochilus xingrensis was high. Conclusion The present methodology meets the technical raquirements of fingerprint and could be a basis for the intrinsic quality control standard for Anoectochilus xingrensis.