中文摘要：

目的：观察戊己丸不同配伍方对平滑肌细胞5-羟色胺34受体及其下游信号通路的影响。方法：采用Bitar的方法分离培养大鼠结肠平滑肌细胞，α-actin免疫组化鉴定。应用免疫荧光法和钙离子探针检测5-羟色胺。受体表达及Ca^2＋浓度；应用ELISA试剂盒检测cAMP，PKA的浓度。结果：1号方和2号方对大鼠结肠平滑肌细胞5-羟色胺3．4受体的表达及Ca^2＋浓度均具有抑制作用（P〈0．05）；可显著下调平滑肌细胞的cAMP和PKA水平（P〈0．05）；2号方作用效果优于1号方。结论：戊己丸可抑制5-羟色胺3，4受体表达及下调Ca^2＋，cAMP，PKA水平。

英文摘要：

Objective：To study the influence of Wuji pill on 5- serotonin（5-HT） downstream signal pathway. Methods： According to Bitar＇ s methods, the rat colon smooth muscle cells （SMC） were isolated and cultured, and then identified by α-actin immunohistochemistry stain. Furthermore, the 5-HT receptor 3, 4 of the SMC membrane and Ca2＋were tested by immunofluorescence stain and calcium ion probe. The cyclic adenosine monophosphate （cAMP） and protein kinase A （PKA） level were measured by ELISA. Results：Two compatibilities can decrease the expression of 5-HT receptor 3,4 and the concentration of Ca2＋ of the SMC significantly（ P 〈 0.05 ） , furthermore down-regulate the level of cAMP and PKA of the SMC significantly（P 〈 0.05）. The effect of NO. 2 Wuji pill was better than No. 1. Conclusion ： Wuji pill can decrease the expression of 5- serotonin receptor 3,4 and down-regulate the level of Ca^2＋ ,cAMP and PKA.