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中文摘要：
多孔硅（PS）具有纳米级尺寸,高的表面积比和生物兼容性为固定生物分子提供了有利的条件。文章采用光电化学腐蚀的方法制备出多孔硅,并将3-氨基丙基三乙氧基硅烷（APTS）共价结合到多孔硅表面实现其生物功能化。通过戊二醛（Gluta）交联的方式将葡萄糖氧化酶（GOD）固定到生物功能化多孔硅上,形成GOD-Gluta-APTS-PS复合结构并用作电化学测量的工作电极。铂金和饱和甘汞电极分别作辅助电极和参比电极。通过测量还原电流对数与电极电势的关系以及计时电流曲线,对10×10^-6-55×10^-6mol dm^-3浓度范围的葡萄糖水溶液进行测量分析,发现还原电流与葡萄糖溶液在一定范围内有线性响应关系。制成的多孔硅酶复合电极间隔5天重复使用1次,20天内性能能保持基本不变。
英文摘要：
Porous silicon （PS） has many advantages, such as nanometer size, large specific surface and bio-compatibility. A porous silicon substrate was obtained by photoelectrochemical etching technique,and then modified to realize biofunctionalization of surface by aminopropyl-triethoxysilane （APTS）. Glucose oxidase （GOD） immobilization was carried out by glutaraldehyde（Gluta） cross-linking to the porous silicon and GOD-Gluta-APTS-PS composite structure was formed. The GOD-Gluta-APTS-PS structure was used as working electrode in a measuring cell sealed by an O-ring. A saturated calomel electrode （SCE） and platinum dish was used as reference electrode and counter electrode, respectively. Chrononamperemeter and logarithmic current-potential measurements were performed to detect a glucose solution. Under the selected conditions,the enzyme composite electrode offered a liner response in the glucose concentration range of 10×10^-6-55×10^-6 mol dm^-3. The sensor remained good response after 20 d through 5 d interval measurements.
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