中文摘要：

瞄准：调查是否与骨头髓 co 有教养的 hepatocytes 的功能间充质的干细胞(MSC ) 能从 acute-on-chronic 肝在浆液被维持失败(ACLF ) 病人。方法：Hepatocyte 支持的功能和从有病毒的肝炎的 18 个病人的 sera 的 cytotoxicity 导致 B 的 ACLF 和 18 个健康志愿者分别地为与 MSC 和 hepatocyte mono-layered 文化 co 有教养的猪的 hepatocytes 被评估。Chemokine 侧面也为正常浆液和肝失败浆液被检验。结果：Hepatocyte 生长因素(HGF ) 和肿瘤坏死因素；肿瘤坏死因素(TNF )- 当表皮的生长因素(EGF ) 和 VEGF 层次显著地被减少时，显著地响应 ACLF 被提高。肝失败浆液样品在 homo-hepatocyte 文化导致了更高的分开率，更低的生存能力和减少的肝支持功能。与 MSC co 有教养的 Hepatocytes 能容忍从 ACLF 病人和吃的类似的肝支持的浆液的 cytotoxicity 在 vitro 与与健康人的浆液有教养的 hepatocytes 相比工作。另外，尽管有， co 有教养的 hepatocytes 维持了一个 proliferative 能力从肝的侮辱失败浆液。结论：ACLF 浆液不在 vitro 损害房间形态学，生存能力，增长和与 MSC co 有教养的 hepatocyte 的全面新陈代谢的能力。

英文摘要：

AIM： To investigate whether the function of hepatocytes co-cultured with bone marrow mesenchymal stem cells （MSCs） could be maintained in serum from acute-on- chronic liver failure （ACLF） patients.METHODS： Hepatocyte supportive functions and cy- totoxicity of sera from 18 patients with viral hepatitis B-induced ACLF and 18 healthy volunteers were evalu- ated for porcine hepatocytes co-cultured with MSCs and hepatocyte mono-layered culture, respectively. Chemo- kine profile was also examined for the normal serum and liver failure serum.RESULTS： Hepatocyte growth factor （HGF） and Tumor necrosis factor; tumor necrosis factor （TNF）-a were re- markably elevated in response to ACLF while epidermal growth factor （EGF） and VEGF levels were significantly decreased. Liver failure serum samples induced a higher detachment rate, lower viability and decreased liver sup- port functions in the homo-hepatocyte culture. Hepato-cytes co-cultured with MSCs could tolerate the cytotoxic- ity of the serum from ACLF patients and had similar liver support functions compared with the hepatocytes cul- tured with healthy human serum in vitro. In addition, co- cultured hepatocytes maintained a proliferative capability despite of the insult from liver failure serum.CONCLUSION： ACLF serum does not impair the cell morphology, viability, proliferation and overall metabolic capacities of hepatocyte co-cultured with MSCs in vitro.