中文摘要：

目的探索IL-6在猪肝细胞与骨髓间充质干细胞（mesenchymal stem cells，MSCs）体外共培养中的作用。方法自中华实验猪髂前上棘抽取骨髓（3只），采用密度梯度离心法分离单个核细胞，贴壁传代培养至第3代；原位两步胶原酶法分离猪肝细胞后与MSCs按照2：1比例使用Millicell小室培养，检测共培养中肝细胞功能，观察IL-6、TNF—α、TGF-α的分泌水平变化。结果肝细胞活率〉95％：共培养组肝细胞自蛋白分泌水平和尿素合成能力均显著高于单纯肝细胞组（P〈0．05）．共培养组IL-6浓度显著高于对照组（P〈0．01）。中和IL-6后共培养体系白蛋白、尿素合成水平明显下降（P〈0．01）：结论MSCs通过分泌IL-6改善共培养体系中肝细胞功能：

英文摘要：

Objective To investigate the role of IL-6 in the co-cultivation system ot porcme hepatocytes with bone marrow mesenehymal stem cells （MSCs） in vitro. Methods Mononuclear cells were isolated from bone marrow aspirate of swines （ n = 3 ） by 1. 077 g/ml density gradient centrifugation. MSCs of passage 3 and primary hepatoeytes harvested by a two-step in situ collagenase perfusion technique were inoculated in Millicell culture inserts at a seeding ratio of 1： 2, and functional changes of heterotypic interactions were characterized. Levels of IL-6, TNF-α, and TGF-α were measured respectively. Results Hepatocyte viability was greater than 95%. Hepatocyte performance levels such as albumin secretion and urea synthesis were all significantly enhanced in co-culture group compared with hepatocyte homo-culture （P 〈 0.05 ）. The IL-6 level also significantly increased in co-culture group （P 〈 0.01 ）. A significant decrease of hepatic functions was observed upon neutralization of IL-6 in co-culture. Conclusion MSC-derived IL-6 plays a key role in the up-regulation of hepatoeyte functions in this co-culture.