中文摘要：

为研究猪繁殖与呼吸综合征病毒（PRRSV）非结构蛋白2（NSP2）之间的差异和生物学特性，本研究根据PRRSV、S1、SY0608株NSP2基因序列保守区设计引物，通过RT—PCR扩增出两毒株的NSP2基因，克隆到pFastBac^TMHTB杆状病毒载体中，将筛选的阳性重组载体pF—S1-NSP2和pF-SY—NSP2，分别转化至含有杆状病毒穿梭载体的DH10Bac感受态大肠杆菌中，经蓝白菌落筛选和PCR鉴定，获得重组表达载体rBac-S1-NSP2和rBac-SY-NSP2。在脂质体介导下转染Sf9昆虫细胞，获得重组杆状病毒rAc-S1-NSP2和rAc-SY—NSP2，Westernblot和间接免疫荧光实验结果表明，本研究成功构建了表达PRRSVS1和SY0608株NSP2蛋白的重组杆状病毒，表达的蛋白与自然感染PRRSV的猪血清均具有良好的反应原性，为进一步研究NSP2蛋白的功能及生物学和免疫学特性奠定了基础。

英文摘要：

The NSP2 genes of PRRSV were amplified by RT-PCR from PRRSV S1 and SY0608 strains, respectively and cloned into the baculovirus donor vector pFastBac^TMHTB. The recombinant plasmids pF-S1-NSP2 and pF-SY-NSP2 DNA were transformed into E.coli competent cells DH10Bac containing baculovirus shuttle vector. The recombinant bacmid （rBac-S1-NSP2 and rBac-SY-NSP2） were screened and verified by PCR. The bacmids were transfected into Sf9 insect cells and two recombinant baculovirus （rAc-S1-NSP2 and rAc-SY-NSP2） were obtained and confirmed by western blot and immunofluorescence assay with antibody against PRRSV. The recombinant NSP2 would be useful resource for studying on the function and immunogenicity of NSP2.