中文摘要：

目的：探讨仪ανβ6整合素介导的细胞与细胞外基质的黏附，及其对氟尿嘧啶诱导的结肠癌细胞凋亡的影响。方法：采用流式细胞仪测定结肠癌细胞株HT-29和WiDr细胞ανβ6整合素的表达，并应用酶联免疫吸附实验（ELISA）及丫啶橙（AO）-溴化乙啶（EB）双荧光染色方法检测细胞凋亡。结果：HT-29细胞、WiDr细胞均表达ανβ6整合素，表达率分别为80．82％及82．96％。氟尿嘧啶诱导结肠癌HT-29和WiDr细胞凋亡。经氟尿嘧啶（20mg／L）作用48h后，酶联免疫吸附实验结果显示，生长于ανβ6整合素配体-纤黏连蛋白（FN）上的HT-29细胞、WiDr细胞的富集系数（EF）分别为1．11±0．04、1．09±0．02，明显低于生长于非整合素配体-多聚赖氨酸（polylisin）上的细胞分别为3．68±0．03、3．72±0．02，P＜0．01；用ανβ6整合素的特异性单抗封闭后再生长于FN上时，HT-29、WiDr细胞的EF分别为2．12±0．04、2．14±0．03，明显高于直接生长于FN上（P＜0．01）。AO—EB双荧光染色法结果显示，生长于FN上的HT-29和WiDr细胞凋亡率分别为（5．6±1．1）％、（5．3±0．7）％，明显低于生长于polylisin上的细胞的凋亡率（37．0±1．4）％、（38．5±0．9）％，P＜0．01；用ανβ6整合素的特异性单抗封闭后再生长于FN上，细胞凋亡率分别为（19．5±1．2）％、（20．0±0．7）％，又有明显提高（P＜0．01）。结论：结肠癌细胞株HT-29和WiDr细胞表达ανβ6整合素，ανβ6整合素介导的细胞与FN的黏附抑制氟尿嘧啶诱导的结肠癌细胞凋亡。提示细胞黏附可能通过抑制细胞凋亡而促使细胞产生耐药性。

英文摘要：

AIM ： To investigate the effects of ctv136 integrin - mediated cell adhesion on 5 - fluorouracil （5 - FU） induced apoptosis in colon carcinoma cell lines. METHODS： The expression of the ανβ6 integrin in colon carcinoma cell lines HT - 29 and WiDr cells was analyzed by flow cytometry. The apoptosis induced by 5 - FU and the effects of ανβ6 integrin - mediated cell adhesion on 5 - FU induced cell apoptosis were measured by enzyme - linked immunosorbent assay （ELISA） method and acridine orange -ethidium bromide （AO -EB） double fluorescent dye staining. RESULTS：Both the colon carcinoma cell lines HT - 29 and WiDr cells expressed the ανβ6 integrin. The percentages of HT - 29 and WiDr cells expression were 80. 82% and 82.96%. 5 - FU induced the apoptosis of colon carcinoma cell lines HT -29 and WiDr. The result of ELISA method displayed that enrichment factor （EF） of HT -29 and WiDr cells planted on fibronectin （FN） -ligand of ανβ6 integrin was lower significantly than the EF of HT -29 and WiDr cells planted on non -integrin ligand polylisin （ 1.11 ± 0. 04 vs 3.68 ± 0. 03, 1.09 ± 0. 02 vs 3.72 ± 0.02, P 〈 0. 01 ） after cultured in medium containing 20 mg/L 5 - FU for 48 h. When HT - 29 and WiDr cells preineubated with ανβ6 integrin blocking antibody were planted on FN again, the EF of HT - 29 and WiDr cells was higher significantly than those directly planted on FN without being blocked by ανβ6 integrin antibody （2. 12 ± 0. 04 vs 1.11±0. 04, 2. 14 ± 0. 03 vs 1.09 ± 0.02, P 〈 0. 01 ）. The AO - EB double fluorescent dye staining displayed that the apoptosis percents of HT - 29 and WiDr cells planting on FN were （5.6 ± 1.1 ） % and （ 5.3 ± 0. 7 ） %, which were lower significantly than those planting on polylisin （ 37.0 ± 1.4） %, （38.5 ± 0. 9） % , P 〈0. 01. When HT -29 and WiDr cells preincubated with ανβ6 integrin blocking antibody were planted on FN again the percents of HT- 29 and WiDr cells apoptosis were （19. 5 ± 1.2）% and （