中文摘要：

目的 观察3T3-L1前脂细胞分化和游离脂肪酸（FFA）对3T3-L1（前）脂肪细胞C5L2基因和蛋白表达的影响。方法采用逆转录（RT）-PCR和流式细胞仪检测不同分化时段和FFA处理后（前）脂肪细胞C5L2mRNA和蛋白表达。结果 3T3-L1脂肪细胞C512mRNA表达呈分化依赖性增加，而C5L2蛋白表达水平在分化早期显著增强，诱导分化6hC5L2蛋白表达增加了21％（61％±18％VS51％±15％，P〈0．05）；分化12h达高峰，增加了38％（70％±12％VS51％±15％，P〈0．01）；分化3d基本恢复至0d水平。在3T3-L1成熟脂肪细胞，0．5mmol／L和1．0mmol／L油酸分别抑制46％（0．58±0．21 vs 1．08±0．46，P〈0．05）和84％（0．18±0．04VS1．08±0．46，P〈0．05）C5L2 mRNA表达，而0．125mmol／L油酸即能显著下调36％（35％±8％vs54％±7％，P〈0．01）C5L2蛋白表达。低浓度棕榈酸均能明显抑制C5L2mRNA和蛋白的表达，1．0mmol／L时c512mRNA和蛋白表达分别减少了41％（0．57±0．28vs0．97±0．41，P〈0．05）和55％（24％±13％VS54％±7％，P〈0．01）。油酸和棕榈酸对前脂肪细胞C512表达差异无统计学意义。结论促酰化蛋白／C5L2途径参与了脂肪细胞分化的调控过程。C512mRNA和蛋白表达的下调可能参与了油酸和棕榈酸诱导的成熟脂肪细胞胰岛素抵抗的发生。

英文摘要：

Objective To observe the changes of expression of the acylation stimulating protein （ASP） functional receptor C5L2 during adipocytes differentiation ,and to study the regulation of C5L2 by free fatty acid （FFA） in cultured adipocytes and preadipocytes. Methods Preadipocytes of the line 3T3-L1 were cultured and induced to differentiate. Oleate or palmitate of the doses 0 - 1.0 mmol/L were added into the cultured fluid of the 3T3-L1 mature adipocytes or preadipocytes overnight. RT-PCR and flow cytometry were used to detect the mRNA and protein expression of C5L2. Results The C5L2 mRNA expression level of the adipocytes 9 days after differentiation was 0.89 ± 0.24, 3.56 times that before induction （0.25 ± 0. 12, P〈0.01 ）. The C5L2 protein expression 6 h after induction was 61% -± 18% , higher by 21% in comparison of that at the 0 d （51% ：i： 15% , P 〈 0.05） , and the C5L2 protein expression 12 h after induction reached a maximal level （70% ± 12% ）, 38% higher than that at 0 d （P 〈0.01）. Then the C5L2 protein expression decreased significantly on the third day after induction to the level on the day 0. The C5L2 mRNA expression levels of the mature adipocytes stimulated by oleate 0. 125, 0.5, and 1.0 mmol/L for 18 h were decreased by 6% , 46% , and 84% respectively compared with that of the oleate 0 mmol/L （all P 〈 0.05 ） ; however, the C5L2 mRNA expression levels of the preadipocytes stimulated by oleate 0. 125, 0.5, and 1.0 mmol/L for 18 h were not significantly different from that of the oleate 0 mmol/L group. The C5L2 mRNA expression levels of the mature adipocytes stimulated by palmitate 0. 125, 0.5, and 1.0 mmol/L for 18 h were decreased suppressed dose-dependently, for example, the C5L2 mRNA expression level of the palmitate 1.0 mmol/L group was lower than that of the palmitate 1.0 mmol/L group by 41% （P 〈0.05） ; however, the C5L2 mRNA expression levels of the palmitate 1.0 mmol/L group was not significantly different from that of the palmitate 0 mmol/L group. Co