中文摘要：

本研究使用CRISPR/Cas9基因编辑技术结合同源重组,以伪狂犬病毒经典疫苗株Bartha-K61为骨架,将其gB基因替换为伪狂犬病毒变异株JS-2012的gB基因,经噬斑纯化、PCR筛选鉴定,获得重组病毒r PRV-BJB。体外生物学特性分析结果显示,重组病毒株r PRV-BJB与疫苗株Bartha-K61具有相似的生长特性和噬斑形态,可作为新型重组伪狂犬病病毒疫苗候选株。

英文摘要：

In this study, we utilized the specific CRISPR/Cas9 gene editing system combined with homologous recombination to replace the gB of Bartha-K61 strain with that of JS-2012 strain. After several rounds plaque purification and PCR-screening, the acquired recombinant virus was identified by sequencing and designated as r PRV-BJB. r PRV-BJB and Bartha-K61 showed similar biological characteristics in vitro, indicating that r PRV-BJB is a promising PRV vaccine candidate that can be performed in following protective efficacy evaluation.