中文摘要：

使用高效毛细管电泳法,对黄花菜中活性化合物芦荟大黄素、山柰酚、绿原酸和槲皮素进行检测。在最佳电泳条件为10 mmol/L Na2B4O7-H3BO3缓冲溶液、pH 9.15、紫外检测波长224 nm、30 kV的外加电压条件下进样5s,4种物质在6 min内实现了分离,4种物质的线性范围分别为0.02-0.21、0.01-0.90、0.01-0.99、0.01-0.90 mg/mL,线性相关系数在0.9976-0.9996之间,检出限分别为2.45×10^-5、1.41×10^-5、1.78×10^-5、8.63×10^-6mg/mL（RSN=3）,平均回收率在97.5%-101.2%之间,相对标准偏差不大于4.76%。实验结果表明该方法快速准确,适合用于黄花菜中活性物质的检测。

英文摘要：

An efficient high performance capillary electrophoresis（HPCE） method was developed for the determination of aloe-emodm,kaempferol,chlorogenic and quercetm in nightlily flowers（Hemerocallis citrina）.Sample injection was carried out by hydrodynamic injection for 5 s at 30 kV.The running buffer was 10 mmol/L Na2B4O7-H3BO3（pH 9.15）,and the detection wavelength was set at 224 nm.Four target compounds were separated in 6 min.Under these optimal conditions,the linear ranges for the above four compounds were 0.02-0.21,0.01-0.90,0.01-0.99,and 0.01-0.90 mg/mL with correlation coefficients between 0.997 6 and 0.999 6,and the limits of detection were 2.45 × 10^-5,1.41 × 10^-5,1.78 × 10^-5 and 8.63 × 10^-6 mg/mL（RSN= 3）,respectively.The average recoveries were 97.5%to 101.2%,with a relative standard deviation（RSD） less than4.76%.This method is fast,accurate and suitable for the detection of these active compounds in H.citrine flowers.