中文摘要：

制备镉离子单克隆抗体,建立快速检测水样中镉离子的间接竞争酶联免疫吸附分析方法（EnzymeLinked Immunosorbent Assay,ELISA）。选择双功能螯合剂1-（4-异硫氰苄基）乙烯基二胺-N,N,N’,N’-四乙酸（ITCBE）,分别与载体蛋白（牛血清白蛋白,BSA;卵清蛋白,OVA）和镉离子连接,制备出免疫原（Cd-ITCBEBSA）和包被抗原（Cd-ITCBE-OVA）,采用免疫原免疫BALB/C小鼠,利用细胞融合杂交瘤技术制备出针对镉离子的单克隆抗体;建立快速检测水样中镉离子的间接竞争ELISA方法。结果：方法的IC_（50）为13.67 ng·m L^（-1）,检测限为0.48 ng·m L^（-1）。该抗体除与汞离子的螯合物的交叉反应率为79.29%之外,与Zn^（2＋）、Cr^（3＋）、Pb^（2＋）、Cu^（2＋）、Ni^（2＋）、Al^（3＋）、Co^（2＋）、Fe^（3＋）、Mg^（2＋）、Ca^（2＋）和Ba^（2＋）均无交叉反应。超纯水、自来水和湖水样品中镉的加标回收率为87.78%～103.3%,相对标准差为1.60%～11.41%。建立的间接竞争酶联免疫吸附法灵敏、快速,可用于水样中镉离子的检测。

英文摘要：

Objective： To prepare monoclonal antibody（m Ab） against cadmium ion and develop an indirect competitive EnzymeLinked Immunosorbent Assay（ELISA） method to rapidly detect the cadmium ion in water samples. Methods： cadmium ion was respectively coupled to carrier protein（bovine serum albumin,BSA; ovalbumin,OVA） via a phenyl isothiocyanate-EDTA（ITCBE） to form the immunogen（Cd-ITCBE-BSA） and coating antigen（Cd-ITCBE-OVA）. BALB / c mice were immunized with Cd-ITCBE-BSA for the preparation of monoclonal antibody（m Ab） against cadmium ion by hybridoma technique.The m Ab-based indirect competitive ELISA for rapidly detecting cadmium ion in water samples was developed. Results： The 50% inhibitory concentration（IC_（50）） was13. 67 ng·m L^（-1）and the detection limit was 0. 48 ng·m L^（-1）.In addition to the cross-reactivity rate of the antibody with mercury ion was 79. 29%,no cross-reactivity was found with Zn^（2＋）,Cr^（3＋）,Pb^（2＋）,Cu^（2＋）,Ni^（2＋）,Al^（3＋）,Co^（2＋）,Fe^（3＋）,Mg^（2＋）,Ca^（2＋）and Ba^（2＋）. Acceptable recovery rates of 87. 78% ～ 103. 3% and relative standard deviation of 1. 60%～11. 41% were obtained in ultra-pure water,tap water and lake water samples. Conclusion： we have developed m Ab-based ELISA for sensitive,rapid detection of cadmium ion in water samples.