中文摘要：

建立了采用超高效液相色谱法（UPLC）测定桦褐孔菌发酵菌丝体中的白桦脂醇、麦角甾醇、羊毛甾醇、胆甾醇、豆甾醇和谷甾醇的质量分数的方法。色谱条件以shim-packXR-ODSⅢ柱（150mm×2mm,2.2μm）进行分离,流动相为乙腈100%,流速为0.8mL/min,检测波长为202nm,柱温为30℃。结果表明,UPLC法具有很好的重复性和回收率。甾类化合物分析的日内和日间相对标准偏差分别为0.10%-0.33%（n=5）和0.07%-1.66%（n=5）。获得了在0.3-1.6μg范围内很好的线性范围。白桦脂醇、麦角甾醇、羊毛甾醇、胆甾醇、豆甾醇和谷甾醇的回收率分为97.05%,98.74%,95.65%,101.45%,96.62%和96.14%。UPLC法适合用来快速、准确定量测定桦褐孔菌发酵菌丝体中6种甾类化合物。

英文摘要：

A ultra performace liquid chromatography（UPLC） method was applied to determinating the contents of betulin,ergosterol,lanosterol,cholesterol,stigmasterol and sitosterol in the submerged-cultured mycelia of Inonotus obliquus.The procedure was carried out on a shim-pack XR-ODSⅢ column（150mm×2mm,2.2μm）,using acetonitrile（100%） as mobile phase with a flow rate of 0.8 m L/min.The detecting wavelength was 202nm and the column temperature was 30℃.This method had good reproducibility and satisfactory recoveries The relative standard deviations of this method were less than 0.10%-0.33%（n=5） and 0.07%-1.66%（n=5） for intraday and interday assays,respectively.A good linear correlation was obtained in a range of 0.3 -1.6μg.The recoveries of betulin,ergosterol,lanosterol,cholesterol,stigmasterol,and sitosterol were 97.05%,98.74%,95.65%,101.45%,96.62% 和 96.14%,respectively.This method could be applied to evaluate real samples,and it was rapid,accurate and suitable for the quantitative determination of the six steroids in the submerged-cultured mycelia of Inonotus obliquus.