中文摘要：

选取14种模型化合物对两种带有不同间隔臂的环糊精键合固定相（Click Alkyl-CD、Click OEG-CD）进行了反相液相色谱模式下的保留行为评价。通过梯度洗脱条件下保留参数计算方法和CSASS软件,根据3次线性梯度的保留值数据,测出14种溶质分子在两种固定相上的保留参数,在此基础上考察流动相含乙腈浓度与保留因子的关系后发现,Click Alkyl-CD和Click OEG-CD在分离非极性和中等极性化合物时主要基于反相液相色谱模式,而某些化合物（如吲唑）在Click OEG-CD上的保留受多种作用力影响,并不基于反相液相色谱模式。疏水性评价结果表明,反相分离模式下Click Alkyl-CD的保留参数和正辛醇-水分配常数的相关性较好（R=0.7）,说明其具有比较强的疏水性;而Click OEG-CD的相关性不高（R〈0.3）,说明疏水作用力以外的其他作用力对化合物在反相模式下的保留影响较大。

英文摘要：

A set of 14 model compounds were selected to evaluate the retention properties of two silica-based cyclodextrin（CD） bonded stationary phases with different spacers（Click Alkyl-CD and Click OEG-CD） under reversed-phase liquid chromatographic（RPLC） mode.The gradient calculation method and CSASS software developed in our group were applied to obtain the retention parameters of the 14 solutes on the two CD bonded stationary phases under 3 gradient conditions.The effect of acetonitrile content in the mobile phase on the retention of both CD stationary phases was studied.It was found that the retention of non-polar and medium polar compounds on the both stationary phases was based on RPLC mode,while the retention of some compounds（such as indazole） on Click OEG-CD was influenced by several kinds of interactions and was not based on RPLC mode.The hydrophobic property of the two CD stationary phases in RPLC mode was studied.High correlation value（R=0.7） between the retention parameter of Click Alkyl-CD and n-octanol-water partition coefficients of the solutes indicated the high hydrophobicity of Click Alkyl-CD.Nevertheless,the low correlation value（R0.3） between the retention parameter of Click OEG-CD and n-octanol-water partition coefficients indicated the presence of influence of other interactions in addition to hydrophobic interaction on the retention in RPLC mode.