中文摘要：

骨头髓的移植间充质的干细胞(BMSC ) 作为治疗外部神经系统的疾病的一个新方法被开发了。当 netrin-1 是为 axonal 路径发现和神经生长的一个批评分子时，它可以也影响脉管的网络形成。这里，我们调查了移植在臀部的神经压碎损害的一个老鼠模型生产 netrin-1 的 BMSC 的效果。我们介绍了臀部的神经压碎损害，然后注射了 1 ×； 10 ⁶ BMSC 由在第二天表示 netrin-1 Ad5-Netrin-1-EGFP 或文化媒介进受伤部分的 recombinant 侵入人体气管粘膜的病菌感染了。在白天 7， 14 和 28 点在注射以后，我们测量了马达神经传导并且检测了 netrin-1 受体 UNC5B 的 mRNA 表情并且删除了在 Colorectal 癌症(两重棉包线) ，和神经营养的因素导出大脑的神经营养的因素(BDNF ) 和由即时 PCR 的神经生长因素(NGF ) 。我们也由西方的弄污的试金检测了 BDNF 和 NGF 的蛋白质表情并且检验了合并了到髓磷脂和 vascellum 的 BMSC。结果证明 Ad5-Netrin-1-EGFP 显著地感染的 BMSC 改进了臀部的神经的函数，并且导致了 BDNF 和 NGF 的增加的表示(P<； 0.05 ) 。而且，在损害以后的 28 天，更多的 Schwann 房间在 Ad5-Netrin-1-EGFP 与控制 BMSC 相比感染的 BMSC 被发现。在结论，生产 netrin-1 的 BMSC 的移植在损害以后改进了臀部的神经的功能。这个方法可以是神经损害的新治疗。

英文摘要：

Transplantation of bone marrow mesenchymal stem cells （BMSCs） has been developed as a new method of treat- ing diseases of the peripheral nervous system. While netrin-1 is a critical molecule for axonal path finding and nerve growth, it may also affect vascular network formation. Here, we investigated the effect of transplanting BMSCs that produce netrin-1 in a rat model of sciatic nerve crush injury. We introduced a sciatic nerve crush injury, and then injected 1×10^6 BMSCs infected by a recombinant adenovirus expressing netrin-1 Ad5-Netrin-l-EGFP or culture medium into the injured part in the next day. At day 7, 14 and 28 after injection, we measured motor nerve con- duction and detected mRNA expressions of netrin-1 receptors UNC5B and Deleted in Colorectal Cancer （DCC）, and neurotrophic factors brain-derived neurotrophic factor （BDNF） and nerve growth factor （NGF） by real-time PCR. We also detected protein expressions of BDNF and NGF by Western blotting assays and examined BMSCs that incorporated into myelin and vascellum. The results showed that BMSCs infected by AdS-Netrin- 1-EGFP significantly improved the function of the sciatic nerve, and led to increased expression of BDNF and NGF （P〈0.05）. Moreover, 28 days after injury, more Schwann cells were found in BMSCs infected by AdS- Netrin-l-EGFP compared to control BMSCs. In conclusion, transplantation of BMSCs that produce netrin-1 improved the function of the sciatic nerve after injury. This method may be a new treatment of nerve injury.