中文摘要：

目的探讨凋亡抑制基因生存素、bcl-2在皮肤鳞状细胞癌（简称鳞癌）皮损及皮肤鳞癌细胞系（SCL-1）细胞中的表达意义。方法细胞免疫组化观察60例鳞癌患者皮损中生存素、bcl-2蛋白的表达并进行统计分析。Western印迹检测HaCaT细胞系和皮肤鳞癌细胞系（SCL-1）细胞中生存素bcl-2蛋白的表达。结果免疫组化示正常皮肤组织中未见bcl-2与生存素表达；生存素、bcl-2在鳞癌皮损中呈阳性表达，bcl-2、生存素蛋白表达阳性率分别为70％、60％，两者表达无相关性（P〉0．05）。生存素的表达与年龄、性别、发病部位、病理分级无明显关系，与有无淋巴结转移密切相关。bcl-2蛋白的表达与年龄、性别、发病部位，淋巴结转移无明显关系，但随着鳞癌肿瘤病理分级的升高而降低。Western印迹分析示生存素、bcl-2蛋白在SCL-1细胞中表达明显高于HaCaT细胞。结论生存素、bcl-2在鳞癌的发生发展中，可能通过不同的抗凋亡路径发挥作用。

英文摘要：

Objective To investigate the expression of survivin and bcl-2 in human squamous cell carcinoma （SCC） lesions and cell line SCL-1. Methods Tissue samples from 60 patients with SCC and 10 normal human controls were immunohistochemically stained to detect the expressions of survivin and bcl-2. Western blot was used to measure the expressions of bcl-2 and survivin proteins in HaCaT human keratinocytes and SCL-1 human squamous cell carcinoma cells. Results In normal control tissues, there was no expressions of survivin or bcl-2, while in SCC, the expression rates of bcl-2 and survivin were 70% and 60%, respectively, and there was no statistical correlation between the expressions of bcl-2 and survivin （P 〉 0.05）. Neither the expression of survivin nor that of bcl-2 was correlated to patients＇ age, gender or lesional site （all P 〉 0.05）. A statistical correlation was observed between the pathological stage in patients and expression of bcl-2 as well as between lymph node metastasis and expression of survivin （both P 〈 0.05）. Western blot analysis revealed a significant increase in the expression of survivin and bcl-2 in SCL-1 cells compared with HaCaT cells. Conclusion In SCC, survivin and bcl-2 seem to play their roles via different anti-apoptotic pathways.