以‘中农8号'黄瓜品种为实验材料,采用营养液栽培法研究了钙对根际低氧胁迫下黄瓜幼苗体内多胺(PAs)含量及多胺氧化酶(PAO)活性的影响.结果表明:(1)各处理黄瓜幼苗根系和叶片中的PAs含量以及3种形态的腐胺(Put)、亚精胺(Spd)、精胺(Spm)含量均表现为低氧高钙(8 mmol·L^-1 Ca2+)>低氧常钙(2 mmol·L^-1 Ca2+)>低氧缺钙(0 mmol·L^-1 Ca2+)>通气常钙(2 mmol·L^-1 Ca2+)处理,而PAO活性却表现出相反的趋势(通气常钙>低氧缺钙>低氧常钙>低氧高钙),且处理间大多存在显著差异(P<0.05);根系中的PAs含量明显高于叶片,而PAO活性明显低于叶片.(2)黄瓜幼苗体内3种形态的PAs以游离态含量最高,其次是结合态,最低为束缚态;游离态和结合态PAs在叶片中均以Spd为主,在根系中均以Put为主,束缚态PAs含量在根系和叶片中均为Spd>Put>Spm.研究表明,在低氧胁迫下,营养液加钙引起黄瓜幼苗体内多胺含量的上升和PAO活性下降,钙参与了黄瓜幼苗体内多胺的代谢过程,对缓解低氧胁迫有重要作用.
The experiment was conducted to study the effects of calcium on polyamines (PAs) contents and polyamine oxidase (PAO) activity in ‘Zhongnong 8' cucumber seedlings under hypoxia stress in hydroponics. The results showed that. (1) the contents of PAs, putrecine (Put), spermidine (Spd) and spermine (Spin) with free,conjugated or bound forms in cucumber seedlings leaves and roots ranked as below,the treatment with hypoxia stress and higher Ca^2+ treatment (8 mmol · L^-1 Ca^2+) was first, the one under hypoxia stress with 2 mmol · L^-1 Ca^2+ followed,hypoxia without calcium one (0 mmol · L^-1 Ca^2+) was the third and one with 2 mmol · L^-1 Ca^2+ was the lowest. At the mean time,PAO activity of above treatments appeared as reserved order,the aeration plant was the highest, hypoxia without calcium one followed, the one with hypoxia stress and 2 mmol · L^-1 Ca^2+ was the third and the one under hypoxia stress with higher Ca^2+ was the lowest (2)The highest contents of three forms of PAs was free PAs,the second was conjugated PAs,the third was bound PAs. These indicated that calcium application increased the content of PAs and decreased the activities of PAO, calcium involved in the process of PAs metabolism and played an important role in alleviating the injury of cucumber seedlings with hypoxia stress.