中文摘要：

目的：探讨体外建立非酒精性脂肪肝细胞模型的方法。方法：体外用MEM培养基培养HepG2细胞,分为模型组和正常组。当细胞融合达到70%-80%,正常组换用新鲜MEM培养液,而模型组改为含有游离脂肪酸（油酸和棕榈酸）混合物的MEM培养基诱导培养24 h。以油红O染色和电镜定性观察细胞内脂滴,同时检测细胞内甘油三酯和丙二醛含量。结果：模型组HepG2细胞内脂滴大量形成,且甘油三酯明显升高,而丙二醛较正常组没有明显升高。结论：采用游离脂肪酸（油酸和棕榈酸）混合物体外诱导HepG2细胞建立的脂肪肝细胞模型,是一种研究细胞脂肪变性简便实用的的方法。

英文摘要：

Objective： To establish an in vitro cell model to investigate hepatic steatosis of non -alcoholic fatty liver disease. Methods： HepG2 cells cultured in MEM containing 10% fetal bovine serum were divided into control group and model group. At 70%-80% confluency,HepG2 cells in model group were exposed to a long-chain mixture of free fatty acids （oleate and palmitate） for 24 h,cells in control group were subject to fresh medium. Lipid droplets were observed with oil red O stain and electron microscope, triglyceride and malonatdehyde were detected by respective assay kits. Results： A large number of lipid droplet were detected in model HepG2 cells; the level of triglyceride increased. However, malonaldehyde did not increase significantly compared with control group. Conclusion： Long chain mixture of free fatty acids （oleate and palmitate） can induce steatosis in HepG2 cells,this in vitro model is simple and pragmatic for study of non -alcoholic fatty liver disease.