中文摘要：

目的探讨T细胞免疫球蛋白黏蛋白结构域分子3（Tim-3）mRNA表达在异基因造血干细胞移植（allo—HSCT）后急性移植物抗宿主病（aGVHD）中的作用。方法回顾性分析100例allo—HSCT患者，根据患者aGVHD状态[0（未发生）～I度aGVHD组、Ⅱ。Ⅳ度aGVHD治疗前组、Ⅱ～Ⅳ度aGVHD治疗好转组]及移植后不同阶段（＋14＋30d、＋31-＋60d、＋61～＋100d）比较各组外周血单个核细胞Tim-3mRNA及血浆IFN-У、IL-2水平。结果0～I度aGVHD患者＋31-＋60dTim-3mRNA相对定量（7．24±2．79）明显高于＋14～＋30d（4．60＋1．66）及＋61～＋100d（3．86±1．36）（P值均〈0．05）；＋14～＋30d、＋31-＋60d、＋61-＋100dII～IV度aGVHD治疗前组的Tim-3mRNA水平分别为9．54±3．05、10．14±3．28、12．82±4．20＋1±30d、＋61±100d，均高于0～I度aGVHD组及治疗好转组；＋31-＋60dⅡ-IV度aGVHD治疗前组明显高于治疗好转组（2．49±0．89），与0～I度aGVHD组（7．24±2．79）相比差异无统计学意义；11～Ⅳ度aGVHD组中Tim一3mRNA水平在不同aGVHD程度、不同脏器之间比较差异均无统计学意义（P〉0．05）。Ⅱ-Ⅳ度aGVHD治疗前组血浆IFN-1、IL-2水平明显高于其他各组，治疗好转组血浆IFN小IL一2水平与0～I度aGVHD组比较差异无统计学意义。结论Tim-3在aGVHD的发生过程中发挥着重要作用。

英文摘要：

Objective To investigate the role of Tim-3 gene expression in acute graft-versus-host disease （aGVHD） after allogeneic hematopoietic stem cell transplantation （allo-HSCT）. Methods Patients after allo-HSCT were retrospectively analyzed, this cohort of patients were divided into different groups according to disease states （grade 0- I aGVHD group, grade II- IV aGVHD group, improved aGVHD group） and time periods （＋ 14-＋30 d, ＋31-＋60 d, ＋61-＋ 100 d） to compare PBMC Tim-3 mRNA expression and plasma IFN-y, IL-2 concentrations among them. Results RT-PCR showed that in grade 0- I aGVHD group, Tim- 3 mRNA expression in patients ＋ 31-＋ 60 d （7.24± 2.79） was significantly higher than that in patients ＋ 14-＋30 d （4.60＋ 1.66） and ＋61-＋ 100 d （3.86± 1.36） （P〈 0.05）. Tim-3 mRNA expressions of grade 11-IV aGVHD group in patients ＋ 14-＋30 d, ＋31-＋60 d, ＋61-＋ 100 d were 9.54± 3.05, 10.14＋3.28, 12.82±4.20, respectively, which in both ＋ 14-＋30 d and ＋61-＋ 100 d were significantly higher than that of grade 0- I aGVHD and improved aGVHD groups （P〈0.05）. In patients ＋31-＋60 d, Tim-3 expression of grade II- IV aGVHD group was higher than improved aGVHD group （2.49±0.89）, while no statistical difference when compared with grade 0- I aGVHD group （7.24±2.79）. In grade I1 -IV aGVHD group, Tim-3 mRNA expression manifested no statistical difference among grades or organ involved （P〉0.05）. ELISA results showed that plasma IFN-y and IL-2 concentrations were higher in gradeII - 1V aGVHD group than of other groups, while no significant difference existed between grade 0- I aGVHD and improved aGVHD groups （P〈0.05）. Conclusion Tim-3 played an important role in the process of aGVHD.